Proteins Of Cupuacu Seeds (theobroma Grandiflorum Schum) And Changes During Fermentation And Roasting [proteínas Da Semente De Cupuaçu E Alterações Devidas à Fermentação E à Torração]

Cupuacu (Theobroma grandiflorum Schum) is a native fruit from northern Brazil with great economic potential. Its productive chain is currently sustained by the pulp market. The seed, treated as a by-product of the pulp industrialization, presents considerably protein content. However, very little or nothing is known on its eletrophoretic profile and amino acids, the main protein fractions and alterations due to the fermentation and toasting. The objective of this study was to characterize protein changes in cupuacu seeds, fermented beans, and roasted beans, through the analysis of the electrophoresis profiles, aminograms, and protein fractionations based on solubility. Fermentation and ro asting promoted a slight reduction in the total protein and amino acid contents. The seeds and fermented beans presented four main protein bands with 15.5, 20.4, 27.1, and 33.6 kDa. The beans submitted to fermentation followed by roasting presented only one strong protein band with an apparent molecular weight of 21.0 kDa. The extractions for protein fractionation based on solubility did not result in pure protein fractions. Four main bands were observed in all isolated protein fractions. The 21.1 kDa band was predominant in all cases. This band is apparently very similar to the albumin fraction of cocoa, whose apparent molecular weight is 21.3 kDa.284986993Official methods of analysis of the Association of Official AOAC (1997) Official methods of analysis of the Association of Official Analytical Chemists, , AOAC, 16 ed. WashingtonAMIN, I., JINAP, S., JAMILAH, B. Vicilin-class globulins and their degradation during cocoa fermentation (1997) Food Chemistry, 59 (1), pp. 1-5BIEHL, B., WEWETZER, C., PASSERN, D., Vacuolar (Storage) proteins of cocoa seeds and their degradation during germination and fermentation (1982) Journal of the Science of Food and Agriculture, 33, pp. 1291-1304BRITO, E.S., Structural and chemical changes in cocoa (Theobroma cacao L) during fermentation, drying and roasting (2001) Journal of the Science of Food and Agriculture, 81 (2), pp. 281-288CARVALHO, A.V., GARCÍA, N.H.P., WADA, J.K.A., Caracterização físico-química e curvas de solubilidade protéica de sementes, amêndoas fermentadas e torradas de cupuaçu (Theobroma grandiflorum Schum). (2005) Brazilian Journal of Food Technology, 8 (2), pp. 127-134COHEN, K.O., JACKIX, M.N.H., Estudo do liquor de cupuaçu. (2005) Ciência e Tecnologia de Alimentos, 25 (1), pp. 182-190DODO, H.W., FRITZ, P.J., FURTEK, D.B., A cocoa 21 kilodalton seed protein has trypsin inhibitory activity (1992) Café Cacao Thé, 36, pp. 279-284GRIMALDI, J., Les possibilités D'amélioration des techniques D'ecabossage et de fermentation dans le processus artisanal de la préparation du cacao. (1978) Café Cacao Thé, 22, pp. 303-316Protein and amino acids (2002) Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids, (PART 2), pp. 1-143. , INSTITUTE OF MEDICINE OF THE NATIONAL ACADEMIES, Washington: The National Academies Press, chap 10, pLAEMMLI, U.K., Clevage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685LERCETEAU, E., Evolution of cacao bean proteins during fermentation: A study by two-dimensional electrophoresis (1999) Journal of the Science of Food and Agriculture, 79 (4), pp. 619-625LOPES, A.S., PEZOA-GARCÍA, N.H., AMAYA-FARFÁN, J., Qualidade nutricional das proteínas de cupuaçu e de cacau. (2008) Ciência e Tecnologia de Alimentos, 28 (2), pp. 1-6PEDÓ, I. Caracterização química e nutricional de novos cultivares de aveia (Avena sativa, L.). Campinas, 1996. 112p. Dissertação - (Mestrado em Ciência da Nutrição), Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas - UNICAMPPETTIPHER, G.L., The extraction and partial purification of cocoa storage proteins (1990) Café Cacao Thé, 34 (1), pp. 23-26SCHAGGER, H., JAGOW, G., Tricine-sodium dodecyl sulfatepolyacrylamide gel 'eletrophoresis for the separation of proteins in the range from 1 to 100 kDa (1987) Analytical Biochemistry, 166, pp. 368-379SCHWAN, R. F. Microbiology of cocoa fermentation: a study to improve quality. In: CONFERÊNCIA INTERNACIONAL DE PESQUISA EM CACAU, 12, Salvador, BA, nov. 1996. Anais... Salvador: CEPLAC, 1996SPACKMAN, D.H., STEIN, W.H., MOORE, S., Automatic recording apparatus for use in the chromatography of amino acids (1958) Analytical Chemistry, 30 (3), pp. 1190-1206. , JuneSPENCER, M.E., HODGE, R., Cloning and sequencing of the cDNA encoding the major albumin of Theobroma cacao (1991) Planta, 183, pp. 528-535SPIES, J.R., Determination of tryptophan in proteins (1967) Analytical Chemistry, 39, pp. 1412-1415. , Washington, vTAI, H., Nucleic acid sequence of a 21 kDa cocoa seed protein with homology to the soybean trypsin inhibitor (Kunitz) family of protease inhibitors (1991) Plant Molecular Biology, 16, pp. 913-915VASCONCELOS, M. A. M. Transformações físicas e químicas durante a fermentação de amêndoas de cupuaçu (Theobroma grandiflorum Schum). Campinas, 1999. 114p. Dissertação - (Mestrado em Tecnologia de Alimentos), Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas - UNICAMPVOIGT, J., BIEHL, B., WAZIR, S.K.S., The major seed proteins of Theobroma cacao L (1993) Journal Food Chemistry, 47, pp. 145-151ZAK, D.K., KEENEY, P.G., Extraction and fractionation of cocoa proteins as applied to several varieties of cocoa beans (1976) Journal of Agricultural and Food Chemistry, 24, pp. 479-482Changes in cocoa proteins during ripening of fruit, fermentation, and further processing of cocoa beans (1976) Journal of Agricultural and Food Chemistry, 24, pp. 483-48