An Esr Study Of Nitrosyl-aplysia Brasiliana Myoglobin And Nitrosyl Annelidae Glossoscolex Paulistus Erythrocruorin

The nitrosyl derivatives of Annelidae Glossoscolex paulistus hemoglobin (an earth worm erythrocruorin (Ec AGp)) and Aplysia brasiliana myoglobin (Mb Apb) are studied using ESR spectroscopy. These two proteins have a quite similar ESR spectra at 100 K, but a different temperature behaviour. The temperature dependence of the nitrosyl Mb Apb spectrum is in good agreement with the Boltzmann distribution. In the case of nitrosyl-Ec AGp, the results are explained by the existence of two types of spectrum in thermodynamic equilibrium, with ΔH = 9.08 kJ/mol, ΔS = 47.15 J/mol and T1 2 = 193 K. There is a great similarity of the nitrosyl-Ec AGp spectra with those reported for elephant myoglobin, suggesting teh presence of the same heme environment with a glutamine residue in the distal site. The pH dependence of the spectrum of nitrosyl-Mb Apb shows that the affinity for nitrosyl binding is higher at high pH (7.3) than at low pH (4.6). The ESR parameters are the same for these two pH values. © 1988.9553315320Kon, (1968) J. Biol. Chem., 243, pp. 4350-4357Kon, (1969) Biochem. Biophys. Res. Commun., 35, pp. 423-427Kon, (1975) Biochim. Biophys. Acta, 379, pp. 103-113Yoshimura, Ozaki, Shintani, Watanabe, (1979) Arch. Biochem. Biophys., 193, pp. 301-313John, Waterman, (1979) FEBS Lett., 106, pp. 219-222Nakai, Hori, Yoshida, Sakamoto, Morimoto, (1978) Biochimica et Biophysica Acta (BBA) - Protein Structure, 532, pp. 17-28Maxwell, Caughey, (1976) Biochemistry, 15, pp. 388-396Dickinson, Symons, (1983) Chem. Soc. Rev., 12, pp. 387-414Morse, Chan, (1980) J. Biol. Chem., 255, pp. 7876-7882Hori, Ikeda-Sato, Yonetani, (1981) J. Biol. Chem., 256, pp. 7849-7855Rossi-Fanelli, Antonini, (1957) Biochimica, 22, pp. 335-344Wittemberg, Briel, Wittemberg, (1965) Biochem. J., 96, pp. 363-373Abe, Meirelles, (1985) Comp. Biochem. Physiol., 80 A, pp. 53-55Ruggiero, Sanches, Tabak, Nascimento, (1986) Canadian Journal of Chemistry, 64, pp. 366-372Bartnicki, Misukami, Homero-Herrera, (1983) J. Biol. Chem., 258, pp. 1599-1602Huber, Epp, Steigman, Formanek, (1971) Eur. J. Biochem., 19, pp. 42-50Say, (1980) Ph.D. Thesis, , Ribeira˜o Preto, Sa˜o PauloMun, Chang, Das, (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 4842-484