Biomass Photochemistry Xiii: Pre-irradiated Lignin From Pinus Radiata D. Don And Its Degradation By Ligninase And Horse-radish Peroxidase

The degradation of dioxane-lignin from Pinus radiata D. Don caused by ligninase and by horse-radish peroxidase under photochemical pretreatment was studied. The photochemical modifications of the dioxanelignin were followed using UV-visible spectroscopy and fluorescence. A rapid decrease in the lignin fluorescence at 400 nm (λexc = 360 nm) after irradiation at different fluences was observed. There is a correlation between the ligninase activity and the photon emission of the enzymatic systems as measured by the oxidation of veratryl alcohol. A high reactivity during the oxidation of lignin at different fluences of irradiation was found by the photon emission technique. The photon emission in lignin degradation by ligninase is similar to that observed in the peroxidase-catalysed oxidation of lignin. Photon emission is an excellent method for measuring the ligninase activity on modified lignin. © 1988.412267273FCT; Fuel Cycle TechnologiesDurán, Gomez, Mansilla, Biomass photo-chemistry. A review and prospects (1987) Polymer Degradation and Stability, 17, p. 131Durán, Mansilla, Gomez, (1983) Annu. Conf. Fis. Quim. Org. (Brazil), 2, p. 152Durán, Mansilla, Biomass Photochemistry. V. Modifications of Lignin by Photochemical Treatment and Its Chemiluminescence (1984) Journal of Macromolecular Science: Part A - Chemistry, 21 A, p. 1467Durán, Mansilla, Gomez, Reyes, Leite, (1984) Actual. Fis. Quim. Org. (Brazil), 3, p. 230Durán, Mansilla, Leite, (1985) Proc. 4th Conf. Fis. Quim. Org., Florianópolis, SC, Brazil, p. 24N. Durán, H. Mansilla, L. C. C. Leite and A. Faljoni-Alario, Enzyme Microbiol. Technol., submitted for publicationFerrer, Durán, (1987) Biotechnol. Lett., 9, p. 361Durán, Mansilla, Reyes, Biomass photochemistry X: Analysis of structural modifications in lignin under UV irradiation (1986) Journal of Photochemistry, 35, p. 209N. Durán, J. Rodríguez, E. Gomez, V. Campos and J. Baeza, Biotechnol. Bioeng., in the pressN. Durán, J.L. Reyes, J. Baeza and V. Campos, Biotechnol. Bioeng., in the pressDurán, Rodríguez, Ferraz, Campos, Chrysonila sitophila (TFB-27441): A hyperlignolytic strain (1987) Biotechnology Letters, 9, p. 357Pepper, Baylis, Adler, THE ISOLATION AND PROPERTIES OF LIGNINS OBTAINED BY THE ACIDOLYSIS OF SPRUCE AND ASPEN WOODS IN DIOXANE–WATER MEDIUM (1959) Canadian Journal of Chemistry, 37, p. 75Campos, Salas, Durán, Rodríguez, Baeza, (1986) Bol. Micol. (Chile), 2, p. 161Tien, Kirk, Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase (1984) Proceedings of the National Academy of Sciences, 81, p. 2280A. Ferraz and N. Durán, Appl. Environ. Microbiol., submitted for publicationHon, (1980) Dev. Polym. Deg., 3, p. 229Kriul'kov, Semenikhina, Kaplin, (1969) Gridokhim. Mater., 52, p. 92Klein, (1970) Am. Chem. Soc. Div. Water Air Waste Chem. Gen. Pap., 10, p. 187Kelin, Rockhill, Eldridge, Park, (1970) Tappi, 53, p. 1469Forsskahl, Product analysis of the photo-oxygenation of tert-butylguaiacylcarbinol sensitized by 4-hydroxy-3-methoxy acetophenone (1984) Journal of Photochemistry, 27, p. 363Gold, Kutsuki, Morgan, OXIDATIVE DEGRADATION OF LIGNIN BY PHOTOCHEMICAL AND CHEMICAL RADICAL GENERATING SYSTEMS (1983) Photochemistry and Photobiology, 38, p. 647Sevestenko, Ermolenko, Karpovich, (1983) Zh. Prikl. Khim. (Leningrad), 56, p. 1196Kutsuki, Enoki, Gold, RIBOFLAVIN-PHOTOSENSITIZED OXIDATIVE DEGRADATION OF A VARIETY OF LIGNIN MODEL COMPOUNDS (1983) Photochemistry and Photobiology, 37, p. 1Gold, Kutsuki, Morgan, OXIDATIVE DEGRADATION OF LIGNIN BY PHOTOCHEMICAL AND CHEMICAL RADICAL GENERATING SYSTEMS (1983) Photochemistry and Photobiology, 38, p. 647Tujereine, Aunins, Reizins, (1984) Khim. Drev., 1, p. 44Timpa, Griffin, (1985) Cellul. Chem. Technol., 19, p. 279Burlakov, Chupka, Enzymatic oxidation of lignin and compounds modeling it. III. Formation of singlet oxygen in the peroxidase oxidation of lignin (1984) Chemistry of Natural Compounds, 20, p. 482Neumann, De Groote, Machado, (1986) Polym. Photochem., 7, p. 40