Toxicity Assay In Kraft E1 Effluent Treated By Ozone: Algae Growth Inhibition And Cytotoxicity In V79 Cells

The efficiency of an ozonation process in the degradation of the Kraft E1 effluent was evaluated. The investigation was focused on the reduction of chemical oxygen demand, total phenols, color and the absorbance at 254 nm. The chronic toxicity was evaluated by growth inhibition of algae Selenatrum capricornutum and cytotoxicity evaluation of effluents samples by determination of the nucleic acid content (NCA) endpoint. After 60 minutes of ozonation, COD and UV254 only reached 21% and 34% efficiency reduction. Total phenols and color were more rapidly removed (at 10 minutes of treatment). The results showed that the chronic toxicity (Selenastrum capricornutum algae) decreases for all effluent doses studied. The cytotoxicity assay indicated that toxic compounds were not generated by the ozonation process. Copyright © 2007 International Ozone Association.2914753Andrews, M.J., Garle, M.J., Clothier, R.H., Reduction of the New Tetrazolium Dye, Alamar Blue, in Cultured Rat Hepatocytes and Liver Fractions (1997) ATLA - Altern. Lab. Anim, 25, pp. 641-653Alvares, A.B.C., Diaper, C., Parsons, A., Partial Oxidation by Ozone to Remove Recalcitrance from Wastewaters - A Review (2001) Environ. Technol, 22, pp. 409-427(1995) Standard Methods for Examination of Water and Wastewater, , American Public Health Association APHA, 19th Ed, Washington, DC: American Public Health AssociationArslan-Alaton, I., The Effect of Pre-ozonation on the Biocompatibility of Reactive Dye Hydrolysates (2003) Chemosphere, 51, pp. 825-833Arslan-Alaton, I., Balcioglu, A., Biodegradability Assessment of Ozonated Raw and Biotreated Pharmaceutical Wastewater (2002) Arch. Environ. Contam. Toxicol, 43, pp. 425-431Arslan-Alaton, I., Coglayar, A.E., Toxicity and Biodegradability Assessment of Raw and Ozonated Procaine Penicillin G Formulation Effluent (2006) Ecotoxicol. Environ. Safety, 63, pp. 131-140Assalin, M.R., Rosa, M.A., Durán, N., Remediation of Kraft Effluent by Ozonation: Effect of Applied Ozone Concentration and Initial pH (2004) Ozone Sci. Eng, 26, pp. 317-322Assalin, M.R., Silva, P.L., Durán, N., Comparaç ão da eficiência do processo de ozonização e ozonização catalítica (MnII e Cu II) na degradaç ão de fenol (2006) Quim Nova, 29, pp. 24-27Atlow, S.C., Bonadonna-Aparo, L., Klibanov, A.M., Dephenolization of Industrial Wastewaters Catalyzed by Polyphenol Oxidase (1984) Biotechnol. Bioeng, 26, pp. 599-603Bajpai, P., Microbial Degradation of Pollutants in Pulp Mill Effluents (2001) Adv. Appl. Microbiol, 48, pp. 79-134Beltran, F.J., Rivas, F.J., Montero-de-Espinosa, R., Ozone Enhanced Oxidation of Oxalic Acid in Water with Cobalt Catalysts. 2. Heterogeneous Catalytic Ozonation (2003) Ind. Eng. Chem. Res, 42, pp. 3218-3224Beltrán, F.J., Rivas, F.J., Montero de Espinosa, R.M., Ozone Enhanced Oxidation of Oxalic Acid in Water (2004) Appl. Catal. B, 47, pp. 101-109Benitez, F.J., Beltran-Heredia, J., Acero, J.L., Rubio, F.J., Rate Reactions of Ozone with Chlorophenols in Aqueous Solutions (2000) J. Hazard. Mater, B79, pp. 271-285Bianchi, V., Fortunati, E., Cellular Effects of an Anionic Surfactant Detected in V79 Fibroblasts by Different Cytotoxicity Assays (1990) Toxicol in Vitro, 4, pp. 9-16Çeçen, F., Investigation of Substrate Degradable and Nonbiodegradable Portion in Several Pulp Bleaching Wastes (1999) Water Sci. Technol, 40, pp. 305-312Chu, W., Ma, C., Quantitative Prediction of Direct and Indirect Dye Ozonation Kinetics (2000) Water Res, 34, pp. 3153-3160Cingi, M.R., De Angelis, I., Fortunati, E., Reggiani, D., Bianchi, V., Tiozzo, R., Zucco, F., Choice and Standardization of Test Protocols in Cytotoxicology: A Multicentre Approach (1991) Toxicol. In Vitro, 5, pp. 119-125Cokgor, E.U., Karahan, O., Arslan-Alaton, I., Saruhan, H., Biological Treatability of Raw and Ozonated Synthetic Penicillin Formulation Effluent (2005) Water Sci. Technol, 52, pp. 89-96De Conti, R., De Oliveira, D.A., Fernandes, A.M.P., Melo, P.S., Rodriguez, J.A., Haun, M., De Castro, S.L., Durán, N., Application of a Multi-Endpoint Cytotoxicity Assay to the Trypanocidal Compounds 2-propen-1-amine Derivatives and Determination of Their Acute Toxicity (1998) In Vitro Mol. Toxicol, 11, pp. 153-160Fontanier, V., Baig, S., Albet, J., Molinier, J., Comparation of Conventional and Catalytic Ozonation for the Treatment of Pulp Mill Wastewater (2005) Environ. Eng. Sci, 22, pp. 127-136Freire, R.S., Kunz, A., Durán, N., Some Chemical and Toxicological Aspects about Paper Mill Effluent Treatment with Ozone (2000) Environ. Technol, 21, pp. 717-721Freire, R.S., Kubota, L.T., Durán, N., Remediation and Toxicity Removal from Kraft E1 Paper Mill Effluent by Ozonation (2001) Environ. Technol, 22, pp. 897-904Gagnon, G.A., Booth, S.D.J., Peldszus, S., Mutti, D., Smith, F., Huck, P.M., Carboxylic Acids: Formation and Removal in Full-Scale Plants (1997) J. Am. Water Works Assoc, 89, pp. 88-97Heuvel, M.R., Ellis, R.J., Tremblay, L.A., Stuthridge, T.R., Exposure of Reproductively Maturing Minbow Trout to a New Zealand Pulp and Paper Mill Effluent (2002) Ecotoxicol. Environ. Saf, 52, pp. 65-75Hostachy, J.C., Lenon, G., Pisicchio, J.L., Coste, C., Legay, C., Reduction of Pulp and Paper Mill Pollution by Ozone Treatment (1997) Water Sci. Technol, 35, pp. 261-268Huber, M.M., Gobel, A., Joss, A., Hermann, N., Loffer, D., McArdell, C.S., Ried, A., von Gurten, U., Oxidation of Pharmaceuticals during Ozonation of Municipal Wastewater Effluents: A Pilot Study (2005) Environ. Sci. Technol, 39, pp. 4290-4299Kunz, A, R.S. Freire, J.J.R. Rohwedder, J.P.R. Gutierrez, and N. Durán, Design and Assembly of an Ozonation System to Production and Utilization of Ozone in a Bench Scale, PI 9802076-5 Brazilian Patent 1998Kunz, A., Reyes, J., Durán, N., New Alternatives on Textile Effluent Remediation (1999) Proc. International Conference on Differential Pollution, pp. 525-531. , Australian Waste Water Association, Perth, Australia, May 16-20Kunz, A., Reginatto, V., Durán, N., Combined Treatment of Textile Effluent using the Sequence Phanerochaete chrysosporium - Ozone (2001) Chemosphere, 44, pp. 281-287Kunz, A., Mansilla, H., Durán, N., A Degradation and Toxicity Study of Three Textile Reactive Dyes by Ozone (2002) Environ. Technol, 23, pp. 911-918Latifoglu, A., Gurol, M.D., The Effect of Humic Acids on Nitrobenzene Oxidation by Ozonation and O3/UV Processes (2003) Water Res, 37, pp. 1879-1889Melo, P.S., Durǎn, N., Haun, M., Derivatives of Dehydrocrotonin, a Diterpene Lactone Isolated from Croton cajucara: Cytotoxicity in Rat Cultured Hepatocytes and in V79 Cells (2002) Hum. Exp. Toxicol, 21, pp. 281-288Melo, P.S., Cavalcante, H.M.M., Barbosa-Filho, J.M., Diniz, M.F.F.M., Medeiros, I.A., Haun, M., Warifteine and Milonine, Alkaloids Isolated from Cissampelos sympodialis Eichl: Cytotoxicity on Rat Hepatocyte Culture and in V79 Cells (2003) Toxicol. Lett, 142, pp. 143-151Melo, P.S., Fabrin-Neto, J.B., Moraes, S.G., Assalin, M.R., Durán, N., Haun, M., Comparative Toxicity of Effluents Processed by Different Treatments in V79 Fibroblasts and Algae Selenastrum capricornutum (2006) Chemosphere, , in pressOchi, T., Suzuki, T., Isono, H., Kaise, T., In Vitro Cytotoxic and Genotoxic Effects of Diphenylarsinic acid, a Degradation Product of Chemical Warfare Agents (2000) Toxicol. Appl. Pharmacol, 200, pp. 64-72Paillard, F., Finot, F., Mouche, I., Prenez, A., Vericat, J.A., Use of Primary Cultures of Rat Hepatocytes to Predict Toxicity in the Early Development of New Chemical Entities (1999) Toxicol. In Vitro, 13, pp. 693-700Pereira, W.S., Freire, R.S., Ferro Zero: Uma nova abordagem para o tratamento de águas contaminadas com compostos orgânicos poluentes (2005) Quim. Nova, 28, pp. 130-136Pokhorel, D., Viraraghavan, T., Treatment of Pulp and Paper Mill Wastewater-A Review (2004) Sci. Total Environ, 333, pp. 37-39Rao, S.S., Quinn, B.A., Burnison, B.K., Hayes, M.A., Metcalfe, C.D., Assessment of the Genotoxic Potential of Pulp Mill Effluent using Bacterial, Fish and Mammalian Assays (1995) Chemosphere, 31, pp. 3553-3566Rodriguez, J.A., Haun, M., Cytotoxicity of Trans-dehydrocrotonin from Croton cajucara on V79 Cells and Rat Hepatocytes (1999) Planta Med, 65, pp. 1-5Sponza, D.T., Application of Toxicity Tests into Discharges of Pulp Paper Industry in Turkey (2003) Ecotoxicol. Environ. Safety, 54, pp. 74-86Sevimli, M.F., Kinaci, C., Decolorization of Textile Wastewater by Ozonation and Fenton's Process (2002) Water Sci. Technol, 45, pp. 279-286Sevimli, M.F., Sarikaya, H.Z., Ozone Treatment of Textile Effluents and Dyes: Effect of Applied Ozone Dose, pH and Dyes Concentration (2002) J. Chem. Technol. Biotechnol, 77, pp. 842-850Tortella, G.R., Diez, M.C., Durán, N., Fungal Diversity and Use in Decomposition of Environmental Pollutants (2005) Crit. Ver. Microbiol, 31, pp. 197-212Vogna, D., Marotta, R., Napolitano, A., Andreozzi, R., d'Ischia, M., Advanced Oxidation of the Pharmaceutical Drug Diclofenac with UV/H 2O2 and Ozone (2004) Water Res, 38, pp. 414-422Zenatais, M.G., Duff, S.J.B., Ozone for Removal of Acute Toxicity from Logyard Run-off (2002) Ozone Sci. Eng, 24, pp. 83-90Zhou, H., Smith, D.W., Process Parameter Development for Ozonation of Kraft Pulp Mill Effluents (1997) Water Sci. Technol, 35, pp. 251-25