Association Between Hla And Leukemia In A Mixed Brazilian Population [associação Entre Hla E Leucemia Em Uma População Brasileira De Etnia Mista]

OBJECTIVE. The main purpose of this study was to investigate the class I HLA antigens and class II HLA allele frequencies in 164 patients with leukemia: 35 patients with ALL (acute lymphoid leukemia), 50 with AML (acute myeloid leukemia) and 78 with CML (chronic myeloid leukemia). METHODS. The genotyping of class I HLA was performed by microlymphocytotoxicity and of class II by PCR-SSP (polymerase chain reaction - sequence specific of primers) (One Lambda, Canoga Park, CA, USA). RESULTS. In patients with LLA, frequencies of HLA-B45 and HLAB56 were higher (P = 0.02; OR = 3.13; 95%IC = 0.94-10.44; P = 0.03; OR = 3.61; 95%IC = 0.47-27.64, respectively), than in controls. In patients with AML, the frequency of HLA-B7 (P = 0.01; OR = 2.41; 95%IC = 1.25-4.67) was higher than in controls. The presence of HLA-B45 (P= 0.01; OR = 3.29; 95%IC = 1.46-7.40), HLA-DRB1*04 (P = 0.002; OR = 2.17; 95%IC = 1.36-3.46) and HLA-DRB1*08 (P = 0.004; OR = 2.36; 95%IC = 1.34-4.16) was associated to increased risk of CML developing. CONCLUSION. Our results suggest that variants of HLA confer susceptibility to the same forms of leukemia, and could provide new tools for the investigation of genetics and etiology of this disease.533252256Bowen, D.T., Etiology of acute myeloid leukemia in the elderly (2006) Semin Hematol, 43 (2), pp. 82-88Yamamoto, M., Figueiredo, V.L.P., Epidemiologia da leucemia linfocítica crônica e leucemia linfocítica crônica familiar (2005) Rev Bras Hematol Hemoter, 27 (4), pp. 229-232Ghodke Y, Joshi K, Chopra A, Patwardhan B. HLA and disease. European BARION LA ET AL. Journal of Epidemiology 200520:475-88Joventino, L.P., Stock, W., Lane, N.J., Daly, K.M., Mick, R., Le Beau, M.M., Certain HLA-DR antigens are associated with specific morphologic and cytogenetic subsets of acute myeloid leukemia (1995) Leukemia, 9 (3), pp. 433-439Visentainer, J.E.L., (2003) Testes prognósticos de rejeição e doença do enxerto contra o hospedeiro em transplantes de células progenitoras hematopoiéticas com doadores HLA-idênticos, , Tese doutorado, Universidade Estadual de Campinas, CampinasSchreuder, G.M., Hurley, C.K., Marsh, S.G.E., Lau, M., Vina-Fernandez, M., Noreen, H.J., The HLA dictionary 2004: A summary of HLA-A, -B, -C, -DRB1/3/4/5 and -DQB1 alleles and their association with serologically defined HLA-A, -B, -C, -DR and -DQ antigens (2005) Tissue Antigens, 65, pp. 1-55Yassukawa, M., Ohminami, H., Kojima, K., Inokuchi, K., Nishimura, Y., Fujita, S., Analysis of HLA-DRB1 alleles in Japanese patients with chronic myelogenous leukemia (2000) American Journal of Hematology, 63, pp. 99-101Mundhada, S., Luthra, R., Cano, P., Association of HLA class I and class II genes with bcr-abl transcripts in leukemia patients with t(9;22) (q34;q11) (2004) BMC Cancer, 4 (25), pp. 1-8Villalobos, C., Rivera, S., Weir-Medina, J., Hassanhi, M., Montiel, M., Gonzales, R., Association of HLA class I and leukemia in Mestizo patients of the state of Zulia, Venezuela (2003) Invest Clin, 44 (4), pp. 283-289Oguz, F.S., Kalayoglu, S., Sarper Diler, A., Tozkir, H., Sargin, D., Carin, M., HLA system affects the age-at-onset in chronic myeloid leukemia (2003) Am J Hematol, 73 (4), pp. 256-262LI, D., Xi, B., Liu, H., Yu, Y., Association of gene HLA-class I with leukemia (2005) Zhongguo Shi Yan Xue Ye Xue Za Zhi, 13 (4), pp. 563-566Chhaya, S.U., Human leukocyte antigens in Indian patients with chronic myeloid leukemia (2006) Leuk Lymphoma, 47 (2), pp. 291-295Cao, H.X., Zhao, L., Zhou, L.X., HLA-DRB1 allele polymorphism associated with susceptibility to leukemia in Han nationality of Gansu (2005) Zhongguo Shi Yan Xue Ye Xue Za Zhi, 13 (5), pp. 788-792Gowans, D., O'Sullivan, A., Rollinson, S., Roddam, P., Groves, M., Fegan, C., Allele and haplotype frequency at human leucocyte antigen class I/II and immunomodulatory cytokine locos in patients with myelodisplasia and acute myeloid leukemia: In search of an autoimmune aetiology (2002) British J Haematol, 117, pp. 541-545Rosas-Cabral, A., Irigoyen, L., Alvarado, L., Vela-Ojeda, J., Ayala, S.M., Tripp-Villanueva, F., HLA Cw3 and HLA Cw4 have a protective effect on acquisition of chronic myeloid leukemia on Mexican patients (2003) Rev Invest Clin, 5 (4), pp. 423-428Khamaganova, E., Aleschenko, S., Murashova, L., Zaretskaya, Y., Immunogenetic factores of predisposition to blood malignancies in Russian population (2001) Russ J Immunol, 6 (3), pp. 265-270Tian, H., Zhou, S.Y., Liu, Z., Fu, Y.G., Lu, F.J., Lin, J.H., Association of HLA - DPB1 alleles with chronic myelogenous leukemia in Southern Chinese hans (2003) Zhongguo Shi Yan Xue Ye Xue Za Zhi, 11 (43), pp. 266-268Barrios, L.M.M., Suárez, V.M., Hernández, A.M.G., Blanco, R.V., Ruiz, M.F., Martinez, M.A., Estudio de los haplotipos HLA en leucemias (1997) Rev Cubana Hematol Inmunol Hemoter, 13 (2), pp. 153-15