Development Of An Analytical Method For Cholesterol Determination In Feed For Ruminants Using Factorial Experimental Design [desenvolvimento De Metodologia Analítica Para Determinaç ão De Colesterol Em Ração Para Ruminantes Através De Planejamento Experimental Fatorial]

A chromatographic method was developed for cholesterol determination in feed for ruminants using response surface methodology. Among the five approaches of sample preparation methods tested, the saponification of the sample without heating presented less interference in the gas chromatography. The method presented a relative standard deviation (RSD) of 4.3%, recoveries between 84 and 87% and detection limit of 0.001 mg of cholesterol per g of feed.31614221426+S1Piironen, V., Lindsay, D.G., Miettinen, T.A., Toivo, J., Lampi, A.M., (2000) J. Sci. Food Agric, 80, p. 939Volin, P., (2001) J. Chromatogr., A, 935, p. 125Van Elswyk, M.E., Schake, L.S., Hargis, P.S., (1991) Poultry Sci, 70, p. 1258Bragagnolo, N., Rodriguez-Amaya, D.B., (2003) J. Food Comp. Anal, 16, p. 147Behrman, E.J., Gopalan, V., (2005) J. Chem. Educ, 82, p. 1791Saldanha, T., Mazalli, M.R., Bragagnolo, N., (2004) Ciênc. Tecnol. Aliment, 24, p. 109Folch, J., Less, M., Stanley, S., (1957) J. Biol. Chem, 226, p. 497Bohac, C.E., Rhee, K.S., Cross, H.R., Ono, K., (1988) J. Food Sci, 5, p. 1642Diemair, W., (1963) Laboratoriumsbuch fur Lebensmittelchemiker, , 8 th ed, Verlag Von Theodor Steinkopff: DresdenRios, A.O., Mercadante, A.Z., (2004) Food Addit. Contam, 21, p. 125Mariutti, L.R.B., Nogueira, G.C., Bragagnolo, N., J. Agric. Food Chem, , no preloMazalli, M.R., Sawaya, A.C.H.F., Eberlin, M.N., Bragagnolo, N., (2006) Lipids, 41, p. 615Box, G.E.P., Hunter, W.G., Hunter, J.S., (1978) Statistics for Experiments: An Introduction to Designs, Data Analysis and Model Building, , Wiley: New YorkDirectiva 2002/657/CE de 12 de agosto de 2002(2002) J. Off. Comun. Europ, L 221, p. 8. , Comissão da União Européia;Ahmida, H.S.M., Bertucci, P., Franzo, L., Massoud, R., Cortese, C., Lala, A., Federici, G., (2006) J. Chromatogr., B: Anal. Technol. Biomed. Life Sci, 842, p. 43Dutta, P.C., Normém, L., (1998) J. Chromatogr., A, 816, p. 177Phillips, K.M., Ruggio, D.M., Bailey, J.A., (1999) J. Chromatogr., B: Anal. Technol. Biomed. Life Sci, 732, p. 17Phillips, K.M., Ruggio, D.M., Toivo, J.I., Swank, M.A., Simpkins, A.H., (2002) J. Food Comp. Anal, 15, p. 123Frega, N., Bocci, F., Lercker, G., (1992) J. Am. Oil Chem. Soc, 69, p. 447Rodriguez-Estrada, M.T., Frega, N., Lercker, G., (2002) Grasas Aceites, 53, p. 76Lagarda, M.J., García-Llatas, G., Farré, R., (2006) J. Pharm. Biomed. Anal, 41, p. 1486Ferrari, A.M., Santos, J., (2005) Ciên. Tecnol. Aliment, 25, p. 132Rossell, J.B., (1991) Analysis of oilseeds, fats and fatty foods, , Elsevier: Londo