Kinetic Modeling Of Cell Death By Shear Stress Of A Hybridoma Culture In A Sparged And Stirred Bioreactor

In animal cell systems, cell death by shear stress is an important factor to be considered when defining the required sparging and agitation to maintain the dissolved oxygen concentration and the homogeneity of the bioreactor. This paper presents the effects of air sparging and agitation in an ideal bioreactor configuration for animal cell culture. In these conditions, the effect of agitation on the cell death rate is negligible and this parameter can be considered directly proportional to the superficial gas rate.49SPEC. ISS.107114Abe, K., Matsuki, N., Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT (2000) Neuroscience Research, 38, pp. 325-329Aloi, L.E., Cherry, R.S., Cellular response to agitation characterized by energy Dissipation at the impeller tip (1996) Chem. Engng Sci., 51 (9), pp. 1523-1529Apenberg, S., Freyberg, M.A., Friedl, P., Shear stress induces apoptosis in vascular smooth muscle cells via an autocrine Fas/FasL pathway (2003) Biochem. Biophys. Res. Commun., 301, pp. 355-359Aunins, J.G., Henzler, H.J., Aeration in cell culture bioreactors (1993) BiotechnologyA Multivolume Treatise, 2nd Edition, pp. 219-281. , eds. Rehm, H. J.Reed, G., Federal Republic of Germany, VCHBliem, R., Katinger, H., Scale-up engineering in animal cell technology: Part II (1988) Tibtech, 6, pp. 224-230Butler, M., The characteristics and growth of cultured cells (1991) Mammalian Cell Biotechnology: A Practical Approach, pp. 1-25. , ed. Butler, M., Great Britain, IRL PressChisti, Y., Animal cell damage in sparged bioreactors (2000) Tibtech, 18, pp. 420-432Chist, Y., Hydrodynamic damage to animal cells (2001) Critical Reviews in Biotechnology, 21 (2), pp. 67-110Feuser, J., Halfar, M., Lütkemeyer, Ameskamp, N., Kula, M.-R., Thömmes, J., Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies (1999) Process Biochemistry, 34, pp. 159-165Gardner, A.R., Gainer, J.L., Kirwan, D.J., Effects of stirring and sparging on cultured hybridoma cells (1990) Biotechnol Bioeng., 35, pp. 940-947Handa-Corrigan, A., Emery, A.N., Spier, R.E., Effect of gas-liquid interfaces on the growth of suspended mammalian cells: Mechanisms of cell damage by bubbles (1989) Enzyme Microb. Technol., 11, pp. 230-235Kunas, T.K., Papoutsakis, E.T., Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment (1990) Biotechnol. Bioeng., 36, pp. 476-483Lash, L.H., Tokarz, J.J., Pegouske, D.M., Susceptibility of primary cultures of proximal tubular and distal tubular cells from rat kidney to chemical induced toxicity (1995) Toxicology, 103, pp. 85-103Leist, C.H., Meyer, H.P., Fiechter, A., A potential and problems of animal cells in suspension (1990) J. Biotechnol., 15, pp. 1-46Léo, P., Ucelli, P., Augusto, E.F.P., Oliveira, M.S., Tamashiro, W.M.S.C., Anti-TNP monoclonal antibodies as reagents for enzyme immunoassay (ELISA) (2000) Hybridoma, 19 (6), pp. 473-479Li, C., Xu, Q., Mechanical stress-initiated signal transductions in vascular smooth muscle cells (2000) Cellular Signalling, 12, pp. 435-445Mardikar, S.H., Niranjan, K., Observations on shear damage to different animal cells in a concentric cylinder viscometer (2000) Biotechnol. Bioeng., 68 (6), pp. 697-704Neter, J., Kutner, M.H., Nachtsheim, C.J., Wasserman, W., (1996) Applied Linear Statistical Models, 4th Edition, pp. 217-241. , WCB/MacGraw-HillPapoutsakis, E.T., Fluid-mechanical damage of animal cells in bioreactors (1991) Tibtech, 9, pp. 427-437Tramper, J., Vlak, J.M., Gooijer, C.D., Scale-up aspects of sparged insect cells bioreactors (1996) Cytotechnology, 20, pp. 221-229Varley, J., Birch, J., Reactor design for large scale suspension animal cell culture (1999) Cytotechnology, 29, pp. 177-205Wagner, A., Marc, A., Engasser, J.M., Einsele, A., The use of Lactate Dehydrogenase (LDH) release kinetics for the evaluation of death and growth of mammalian cells in perfusion reactors (1992) Biotechnol. Bioeng., 39, pp. 320-326Wu, J., Mechanisms of animal cell damage associated with gas bubbles and cell protection by medium additives (1995) J. Biotechnol., 43, pp. 81-9