In Vitro Propagation Of Sacha Inchi [propagação In Vitro De Sacha Inchi]

The aim of this study was to perform an in vitro evaluation of the auxin:cytokinine ratio in different segments of the epicotyl and hypocotyl of Sacha inchi (Plukenetia Volubilis Linneo) seeds germinated in vitro. The segments apical (A), median (B) and basal (C) were introduced into semi-solid MS culture medium (2.0g L -1 Phytagel), supplemented with MS vitamins, sucrose (30.0g L -1) and submitted to three doses of auxin indolebutyric acid - IBA (0; 0.1; 0.5mg L -1), associated with four doses of the cytokinine benzylaminopurine - BAP (0; 0.1; 0.5; 1.0mg L -1), totaling 36 treatments. After nine weeks of in vitro cultivation, the apical segment (A) presented shoot formation by direct organogenesis at the concentrations of 0.5 and 1.0 of BAP associated with 0.0 and 0.1 of IBA. It is feasible to use in vitro cultivation with the apical region of seeds germinated in vitro used as explants.42711681172Céspedes, E.I.M., (2006) Cultivo de Sacha Inchi, p. 11. , Tarapoto, San Martin, Peru: INIIA, Subdirección De Recursos Geneticos Y BiotecnologíaDimech, A.M., Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia (2007) Plant Cell, Tissue and Organ Culture, 88, pp. 157-165. , http://www.springerlink.com/content/k070lrjll72x370g/fulltext.html, 9188. Disponível em:,. Acesso em: 02 fev. 2007. doi: 10.1007/s11240-006--1Guillén, M.D., Characterization of Sacha Inchi (Plukenetia volubilis L.) Oil by FTIR Spectroscopy and 1H NMR. Comparison with Linseed Oil (2003) Journal of the American Oil Chemist Society, 80, pp. 755-762Huamán, J., Efecto de la Plukenetia volubilis Linneo (Sacha inchi) en la trigliceridemia posprandial (2008) Annales Faculdad Medicina, 69, pp. 263-266Jesus, A.M.S., Cultivo in vitro de embriões zigóticos de Jatropha (2003) Revista Ceres, 50, pp. 183-189Lima, G.P.P., Efeito do BAP e ANA e atividade da peroxidase em mandioca (Manihot esculenta Crantz cv Mcol 22) cultivada in vitro (2002) Revista Brasileira de Agrociência, 8 (2), pp. 127-130Mala, J., Role of phytohormones in organogenic ability of elm multiplicated shoots (2005) Biologia Plantarum, 50, pp. 8-14Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Physiologia Plantarum, 15, pp. 473-497Nunes, C.F., Diferentes suplementos no cultivo in vitro de embriões de pinhão manso (2008) Pesquisa agropecuária brasileira, 43 (1), pp. 9-14Vazquez Molina, D.E., Sugar cane buds as an efficient explant for plantlet regeneration (2005) Biologia Plantarum, 49, pp. 481-485. , http://springerlink.com/content/l165h14730u55466/, 2005. Disponível em:,. Acesso em: 03 se