Redescription Of Physalaemus Barrioi (anura: Leiuperidae)

Physalaemus barrioi is a poorly known frog endemic to the highlands of Serra da Bocaina in the Atlantic Forest of southeastern Brazil. Herein, we redescribe the species based on the type series and new specimens collected at the type locality. We also present additional data on its vocalization, karyotype, and tadpole, including the external morphology, internal oral features, and chondrocranium. Physalaemus barrioi can be diagnosed by the following combination of characters: SVL in males larger than 24 mm; inguinal glands associated with dark ocelli; throat and vocal sac dark brown; and chest dark with small light blotches. Bioacoustical characteristics also distinguish P. barrioi from all other species of Physalaemus. The tadpole of P. barrioi is very similar to those of the P. gracilis group, from which it can be distinguished by several characteristics, including marginal papillae in a single row, absence of submarginal papillae, elliptical nares, and upper jaw sheath in a wide arch. © 2012 by the American Society of Ichthyologists and Herpetologists.3507518Alcalde, L., Natale, G.S., Cajade, R., The tadpole of Physalaemus fernandezae (Anura: Leptodactylidae) (2006) Herpetological Journal, 16, pp. 203-211Altig, R., McDiarmid, R.W., Body plan: Development and morphology (1999) Tadpoles: The Biology of Anuran Larvae, pp. 24-51. , R. W. McDiarmid and R. Altig (eds.). University of Chicago Press, ChicagoAmaral, J.L.V., Cardoso, A.J., Recco-Pimentel, S.M., Cytogenetic analysis of three Physalaemus species (Amphibia, Anura) (2000) Caryologia, 53, pp. 283-288Ananias, F., Mendes, S.M., Silva, A.P.Z., Caracterização cromosso○mica e padrão de bandamento C de Physalaemus moreirae (Anura, Leptodactylidae) (2005) Anais Do 51u Congresso Brasileira de Genética, p. 426Baêta, D., Lourenço, A.C.C., Nascimento, L.B., Tadpole and advertisement call of Physalaemus erythros Caramaschi, Feio and Guimarães-Neto, 2003 (Amphibia, Anura, Leiuperidae) (2007) Zootaxa, 1623, pp. 39-46Baêta, D., Lourenço, A.C.C., Pezzuti, T.L., Pires, M.R.S., The tadpole, advertisement call, and geographic distribution of Physalaemus maximus Feio, Pombal and Caramaschi, 1999 (Amphibia, Anura, Leiuperidae) (2007) Arquivos Do Museu Nacional, 65, pp. 27-32Barrio, A., El género Physalaemus (Anura, Leptodactylidae) en la Argentina (1965) Physis, 25, pp. 70421-70444Beçak, M.L., Chromosomal analysis of eighteen species of Anura (1968) Caryologia, 21, pp. 191-208Bokermann, W.C.A., Três novas espécies de Physalaemus do sudeste brasileiro (Amphibia, Leptodactylidae) (1967) Revista Brasileira de Biologia, 27, pp. 135-143Borteiro, C., Kolenc, F., Redescription of the tadpoles of three species of frogs from Uruguay (Amphibia: Anura: Leiuperidae and Leptodactylidae), with notes on natural history (2007) Zootaxa, 1638, pp. 1-20Both, C., Kwet, A., Solé, M., The tadpole of Physalaemus lisei Braun and Braun, 1977 (Anura, Leptodactylidae) from southern Brazil (2006) Alytes, 24, pp. 109-116Brum-Zorrilla, N., Saez, F.A., Chromosomes of Leptodactylidae (Amphibia anura) (1968) Experientia, 24, p. 969Cannatella, D., Architecture: Cranial and axial skeleton (1999) Tadpoles: The Biology of Anuran Larvae, pp. 52-91. , R. W. McDiarmid and R. Altig (eds.). University of Chicago Press, ChicagoCardoso, A.J., Biologia reprodutiva de Physalaemus cuvieri Fitz., 1826 (Amphibia, Anura), na natureza (1981) Ciência e Cultura, 33, pp. 1224-1228Cardoso, A.J., Haddad, C.F.B., Nova espécie de Physalaemus do grupo signiferus (Amphibia, Anura, Leptodactylidae) (1985) Revista Brasileira de Biologia, 45, pp. 33-37Cassini, C.S., Cruz, C.A.G., Caramaschi, U., Taxonomic review of Physalaemus olfersii (Lichtenstein & Martens, 1856) with revalidation of Physalaemus lateristriga (Steindachner 1864) and description of two new related species (Anura: Leiuperidae) (2010) Zootaxa, 2491, pp. 1-33Cei, J.M., Amphibians of Argentina (1980) Monitore Zoologico Italiano (NS) Monografia, 2, pp. 1-609Cocroft, R.B., Ryan, M.J., Patterns of advertisement call evolution in toads and chorus frogs (1995) Animal Behaviour, 49, pp. 283-303Conte, C.E., Nomura, F., Rossa-Feres, D.C., D'Heursel, A., Haddad, C.F.B., The tadpole of Scinax catharinae (Anura: Hylidae) with description of the internal oral morphology, and a review of the tadpoles from the Scinax catharinae group (2007) Amphibia-Reptilia, 28, pp. 177-192Cruz, C.A.G., Pimenta, B.V.S., A new species of Physalaemus Fitzinger, 1826 from southern Bahia, Brazil (Anura: Leptodactylidae) (2004) Journal OfHerpetology, 38, pp. 480-486De Lucca, E.J., Jim, J., Foresti, F., Chromosomal studies in twelve species of Leptodactylidae and one Brachycephalidae (1974) Caryologia, 27, pp. 183-191Duellman, W.E., (2001) Hylid Frogs of Middle America, , Society of the Study of Amphibians and Reptiles/Natural History Museum of the University of Kansas, Ithaca, New YorkFabrezi, M., Vera, R., Caracterización morfoló gica de larvas de anuros del noroeste argentino (1997) Cuadernos de Herpetología, 11, pp. 37-49Frost, D.R., (2011) Amphibian Species of the World: An Online Reference, , http://research.amnh.org/vz/herpetology/amphibia/, Version 5.5 (31 January 2011), New YorkGarcia, P.C.A., Sawaya, R.J., Martins, I.A., Brasileiro, C., Verdade, V., Jim, J., Segalla, M.V., Araújo, O.G.S., Anfíbios (2009) Fauna Ameaçada de Extinção Do Estado de São Paulo, pp. 329-347. , P. M. Bressan, M. C. M. Kierulff, and A. M. Sugieda (eds.). Fundação Parque Zooló gico de São Paulo/Secretaria do Meio Ambiente, São Paulo, BrazilGiaretta, A.A., Menin, M., Reproduction, phenology and mortality sources of a species of Physalaemus (Anura: Leptodactylidae) (2004) Journal of Natural History, 38, pp. 1711-1722Gomes, F.B.R., Provete, D.B., Martins, I.A., The tadpole of Physalaemus Jordanensis Bokermann, 1967 (Anura, Leiuperidae) from Campos do Jordão, Serra da Mantiqueira, southeastern Brazil (2010) Zootaxa, 2327, pp. 65-68Gosner, K.L., A simplified table for staging anuran embryos and larvae with notes on identification (1960) Herpetologica, 16, pp. 183-190Green, D.M., Sessions, S.K., Nomenclature for chromosomes (1991) Amphibian Cytogenetics and Evolution, pp. 431-432. , D. M. Green and S. K. Sessions (eds.). Academic Press, San DiegoHaas, A., Phylogeny of frogs as inferred from primarily larval characters (Amphibia: Anura) (2003) Cladistics, 19, pp. 23-89Haddad, C.F.B., Sazima, I., A new species of Physalaemus (AmphibiaLeptodactylidae) from the Atlantic forest in southeastern Brazil (2004) Zootaxa, 479, pp. 1-12Heyer, W.R., Rand, A.S., Cruz, C.A.G., Peixoto, O.L., Nelson, C.E., Frogs of Boracéia (1990) Arquivos de Zoologia, 31, pp. 231-410Heyer, W.R., Wolf, A.J., Physalaemus crombei (Amphibia: Leptodactylidae), a new frog species from Espirito Santo, Brazil with comments on the P. signifer group (1989) Proceedings of Biological Society of Washington, 102, pp. 500-506Howell, W.M., Black, D.A., Controlled silverstaining of nucleolus organizer regions with a protective colloidal developer: A 1-step method (1980) Experientia, 36, pp. 1014-1015Kehr, A.I., Schaffer, E.F., Duré, M.I., The tadpole of Physalaemus albonotatus (Anura: Lepodactylidae) (2004) Journal of Herpetology, 38, pp. 145-148King, M., C-banding studies in Australian hylid frogs: Secondary constriction structure and the concept of euchromatin transformation (1980) Chromosoma, 80, pp. 191-207Kolenc, F., Borteiro, C., Tedros, M., Nu'Ñez, D., Maneyro, R., The tadpole of Physalaemus henselii (Peters) (Anura: Leiuperidae) (2006) Zootaxa, 1360, pp. 41-50Langone, J.A., Descripción de la larva de Physalaemus gracilis (Boulenger, 1883) (Amphibia, Anura, Leptodactylidae) (1989) Comunicaciones Zoologicas Del Museo de Histó Ria Natural de Montevideo, 12, pp. 1-10Lynch, J.D., Evolutionary relationships, osteology, and zoogeography of leptodactyloid frogs (1971) Miscellaneous Publication, Museum of Natural History, University of Kansas, 53, pp. 1-238Miranda, N.E.D.O., Ferreira, A., Morfologia oral interna de larvas dos gêneros Eupemphix, Physalaemus e Leptodactylus (Amphibia: Anura) (2009) Biota Neotropica, 9, pp. 165-176Nascimento, J., Quinderé, Y.R.S.D., Recco- Pimentel, S.M., Lima, J.R.F., Lourenço, L.B., Heteromorphic Z and Wsex chromosomes in Physalaemus ephippifer (Steindachner, 1864) (Anura, Leiuperidae) (2010) Genetica, 138, pp. 1127-1132Nascimento, L.B., Caramaschi, U., Cruz, C.A.G., Taxonomic review of the species groups of the genus Physalaemus Fitzinger, 1826 with revalidation of the genera Engystomops Jiménes-de- la-Espada, 1872 and Eupemphix Steindachner, 1863 (Amphibia, Anura, Leptodactylidae) (2005) Arquivos Do Museu Nacional, 63, pp. 297-320Nascimento, L.B., Carvalho, R.R., Wogel, H., Fernandes, D.S., Feio, R.N., Reprodução e descrição do girino de Physalaemus rupestris Caramaschi, Carcerelli and Feio, 1991 (Amphibia, Anura, Leptodactylidae) (2001) Boletim Do Museu Nacional, 450, pp. 1-10Nascimento, L.B., Verdade, V., Physalaemus barrioi (2004) IUCN 2010. IUCN Red List of Threatened Species, , www.iucnredlist.org, Version 2010.1. Downloaded on 16 March 2010Nomura, F., Rossa-Feres, D.C., Prado, V.H.M., The tadpole of Physalaemus fuscomaculatus (Anura: Leptodactylidae), with a description of internal oral morphology (2003) Zootaxa, 370, pp. 1-8Oliveira, M.I.R.R., Weber, L.N., Ruggeri, J., The tadpole of Physalaemus albifrons (Spix, 1824) (Anura, Leiuperidae) (2010) South American Journal of Herpetology, 5, pp. 249-254Perotti, M.G., Céspedez, J.A., The tadpole of Physalaemus santafecinus, with comments on buccopharyngeal morphology (1999) Journal of Herpetology, 33, pp. 312-315Pimenta, B.V.S., Cruz, C.A.G., The tadpole and advertisement call of Physalaemus aguirrei Bokermann, 1966 (Amphibia, Anura, Leptodactylidae) (2004) Amphibia- Reptilia, 25, pp. 197-204Pimenta, B.V.S., Cruz, C.A.G., Silvano, D.L., A new species of the genus Physalaemus Fitzinger, 1826 (Anura, Leptodactylidae) from the Atlantic rain forest of southern Bahia, Brazil (2005) Amphibia-Reptilia, 26, pp. 201-210Provete, D.B., Garey, M.V., Dias, N.Y.N., Rossa-Feres, D.C., The tadpole of Physalaemus moreirae (Anura: Leiuperidae) (2011) Herpetologica, 67, pp. 258-270Quinderé, Y.R.S.D., Lourenço, L.B., Andrade, G.V., Tomatis, C., Baldo, D., Recco-Pimentel, S.M., Polytypic and polymorphic cytogenetic variations in the widespread anuran Physalaemus cuvieri (Anura, Leiuperidae) with emphasis on nucleolar organizing regions (2009) Biological Research, 42, pp. 79-92Rossa-Feres, D.C., Jim, J., Tadpole of Physalaemus centralis (Anura, Leptodactylidae) (1993) Copeia, 1993, pp. 566-569Rossa-Feres, D.C., Jim, J., Fonseca, M.G., Diets of tadpoles from a temporary pond in southeastern Brazil (Amphibia, Anura) (2004) Revista Brasileira de Zoologia, 21, pp. 745-754Schmid, M., Chromosome banding in amphibia II: Constitutive heterochromatin and nucleolus organizers regions in Ranidae, Microhylidae and Rhacophoridae (1978) Chromosoma, 68, pp. 131-148Schmid, M., Olert, J., Klett, C., Chromosome banding in Amphibia. III. Sex chromosomes in Triturus (1979) Chromosoma, 71, pp. 29-55Silva, A.P.Z., Júnior, F.A.B., Haddad, C.F.B., Kasahara, S., Karyotypes and nucleolus organizer regions in four species of the genus Physalaemus (Anura, Leptodactylidae) (2000) Iheringia, 88, pp. 159-164Sokol, O.M., The larval chondrocranium of Pelodyes punctatus, with a review of tadpole chondrocrania (1981) Journal of Morphology, 169, pp. 161-183Taylor, R., Van Dyke, G.C., Revised procedures for staining and clearing small fishes and other vertebrates for bone and cartilage study (1985) Cybium, 9, pp. 107-112Toledo, L.F., Haddad, C.F.B., Colors and some morphological traits as defensive mechanisms in anurans (2009) International Journal of Zoology, pp. 1-12. , 910892Tomatis, C., Baldo, D., Kolenc, F., Borteiro, C., Chromosomal variation in the species of the Physalaemus henselii group (Anura: Leiuperidae) (2009) Journal of Herpetology, 43, pp. 555-559Vera Candioti, M.F., Anatomy of anuran tadpoles from lentic water bodies: Systematic relevance and correlation with feeding habits (2007) Zootaxa, 1600, pp. 1-175Vittorazzi, S., Lourenço, L., Del-Grande, M., Recco- Pimentel, S., Satellite DNA derived from 5S rDNA in Physalaemus cuvieri (Anura, Leiuperidae) (2011) Cytogenetic and Genome Research, 134, pp. 101-107Wassersug, R.J., Oral morphology of anuran larvae: Terminology and general description (1976) Occasional Papers of the Natural History Museum of the University of Kansas, 48, pp. 1-23Weber, L.N., Carvalho-E-Silva, S.P., Gonzaga, L.P., The tadpole of Physalaemus soaresi Izecksohn, 1965 (Anura: Leptodactylidae), with comments on taxonomy, reproductive behavior, and vocalizations (2005) Zootaxa, 1072, pp. 35-42Weber, L.N., Gonzaga, L.P., Carvalho-E-Silva, S.P., A new species of Physalaemus Fitzinger, 1826 from the lowland Atlantic Forest of Rio de Janeiro State, Brazil (Amphibia, Anura, Leptodactylidae) (2005) Arquivos Do Museu Nacional, 63, pp. 677-684Wogel, H., Abrunhosa, P.A., Pombal, J.P., Atividade reprodutiva de Physalaemus signifer (Anura, Leptodactylidae) em ambiente temporário (2002) Iheringia, 92, pp. 57-7