Oxidative Damage Of Mitochondria Induced By Fe(ii)citrate Or T-butyl Hydroperoxide In The Presence Of Ca2+: Effect Of Coenzyme Q Redox State

The role of coenzyme Q on the process of mitochondrial membrane damage associated with oxidative stress was studied in a suspension of uncoupled mitochondria exposed to Ca2+ in the presence of Fe(II)citrate or t-butyl hydroperoxide. Reduction of coenzyme Q by succinate was shown to inhibit both inner membrane lipid peroxidation and permeabilization induced by Fe(II)citrate. In contrast, the inner membrane permeabilization induced by Ca2+ alone or Ca2+ plus t-butyl hydroperoxide was potentiated by the presence of succinate. These results support our previous proposition that the mitochondrial damage associated with oxidative stress generated by these prooxidants in the presence of Ca2+ is mediated by different mechanisms. © 1994.1815559Boveris, Oshino, Chance, The cellular production of hydrogen peroxide (1972) Biochem. J., 128, pp. 617-630Reed, Review of the current status of calcium and thiols in cell injury (1990) Chemical Research in Toxicology, 5, pp. 495-502Vercesi, Hoffmann, Generation of reactive oxygen metabolites and oxidative damage in mitochondria: The role of calcium (1993) Methods in toxicology: “Mitochondrial Dysfunction”, 2, pp. 256-265. , D.P. Jones, L.H. Lash, Academic Press, New YorkCadenas, Boveris, Ragan, Stoppani, Production of superoxide radicals and hydrogen peroxide by NADH-ubiquinone reductase and ubiquinol-cytochrome c reductase from beef-heart mitochondria (1977) Arch. Biochem. Biophys., 180, pp. 248-257Beyer, The participation of coenzyme Q in free radical production and antioxidation (1990) Free Radic. Biol. Med., 8, pp. 545-565Forsmark, Aberg, Norling, Nordenbrand, Dallner, Ernster, Inhibition of lipid peroxidation by ubiquinol in submitochondrial particles in the absence of Vitamine E (1991) FEBS Lett, 285, pp. 39-43Bindoli, Lipid peroxidation in mitochondria (1988) Free Radical Biology and Medicine, 5, pp. 247-261Gunter, Pfeiffer, Mechanisms by which mitochondria transport calcium (1990) Am. J. Physiol., 258, pp. C755-C786Lehninger, Vercesi, Bababunmi, Regulation of Ca2+ release from mitochondria by the oxidation reduction state of pyridine nucleotides (1978) Proc. Natl. Acad. Sci. USA, 75, pp. 1690-1694Crompton, Ellinger, Costi, Inhibition by cyclosporin A of a Ca2+-dependent pore in heart mitochondria activated by inorganic phosphate and oxidative stress (1988) Biochem. J., 255, pp. 357-360Fagian, Pereira-da-Silva, Martins, Vercesi, Membrane protein thiol cross-linking associated with the permeabilization of the inner mitochondrial membrane by Ca2+ plus prooxidants (1990) J. Biol. Chem., 265, pp. 19955-19960Valle, Fagian, Parentoni, Meinicke, Vercesi, The participation of reactive oxygen species and protein thiols in the mechanism of mitochondrial inner membrane permeabilization by calcium plus prooxidants (1993) Arch. Biochem. Biophys., 307, pp. 1-7Szabo, Zoratti, The mitochondrial megachannel is the permeability transition pore (1992) Journal of Bioenergetics and Biomembranes, 24, pp. 111-117Castilho, Meinicke, Almeida, Hermes-Lima, Vercesi, Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins (1994) Arch. Biochem. Biophys., 308, pp. 158-163Pedersen, Greenalwalt, Reynafarje, Hullihen, Decker, Soper, Bustamante, Preparation and characterization of mitochondria and liver derived tissue (1978) Methods in Cell Biol., 20, pp. 411-481Boveris, Cadenas, Stoppani, Role of ubiquinone in the mitochondrial generation of hydrogen peroxide (1976) Biochem. J., 156, pp. 435-444Buege, Aust, Microsomal lipid peroxidation (1978) Methods in enzymology, 52, pp. 302-310. , S. Fleisher, L. Packer, Academic Press, New YorkHalliwell, Gutteridge, Lipid peroxidation: A radical chain reaction (1989) Free radicals in biology and medicine, pp. 234-260. , B. Halliwell, J.M.C. Gutteridge, Clarendon Press, OxfordErdahl, Krebsbach, Pfeiffer, A comparison of phospholipid degradation by oxidation and hydrolysis during the mitochondrial permeability transition (1991) Arch. Biochem. Biophys., 285, pp. 252-260Beyer, Nordenbrand, Ernster, The function of coenzyme Q in free radical production and as an antioxidant: A review (1987) Chem. Scripta, 27, pp. 145-153Svingen, Buege, O'Neal, Aust, The mechanism of NADPH-dependent lipid peroxidation: The propagation of lipid peroxidation (1979) J. Biol. Chem., 254, pp. 5892-5899Cadenas, Boveris, Enhancement of hydrogen peroxide formation by protophores and ionophores in antimycin-supplemented mitochondria (1980) Biochem. J., 188, pp. 31-37Stadtman, Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions (1993) Annu. Rev. Biochem., 62, pp. 797-82