Erythrocruorin Of Glossoscolex Paulistus (righi) (oligochaeta, Glossoscolecidae): Effects Of Divalent Ions, Acid-alkaline Transition And Alkali And Urea Denaturation

The oxygen affinity and cooperativity of erythrocruorin of Glossoscolex paulistus were studied as a function of cation concentration (Ca2+, Mg2+ and Mn2+). Acid-alkaline transition showed the presence of population multiplicity. Differences in t 1 2 values were observed for native and dissociated forms when submitted to alkaline medium. Urea 6.0-8.0 M caused modifications of the protein structure. © 1995.1112311318Abe, Meirelles, Oxygen binding properties of erythrocruorin and blood pH of the giant earthworm Glossoscolex paulistus (Oligochaeta, Glossoscolecidae) (1985) Comp. Biochem. Physiol., 80, pp. 53-55Antonini, Brunori, (1971) Haemoglobin and Myo-globin in Their Reactions with Ligands, , North-Holland, AmsterdamCaracelli, Meirelles, Tabak, Baffa Filho, Nascimento, An ESR study of nitrosyl—Aplysia brasiliana myoglobin and nitrosyl annelidae Glossoscolex paulistus erythrocruorin (1988) Biochim. Biophys. Acta, 955, pp. 315-320Costa, Bonafé, Meirelles, Galembeck, Sedimentation coefficient and minimum molecular weight of extracellular hemoglobin of Glossoscolex paulistus (Oligochaeta, Glossocolecidae) (1988) Brazilian J. Med. Biol. Res., 21, pp. 115-118Desideri, Verzili, Ascoli, Chiancone, Antonini, High and low-spin EPR forms of the ferric derivative of earthworm erythrocruorin (1982) Biochim. Biophys. Acta, 708, pp. 1-5Fushitani, Imai, Riggs, Oxygenation properties of hemoglobin from the earthworm Lumbricus terrestris (1986) J. biol. Chem., 261, pp. 8414-8423Giardina, Chiancone, Antonini, Studies on erythrocruorin III. Oxygen equilibrium of earthworm erythrocruorin (1975) J. mol. Biol., 93, pp. 1-10Haeser, Datadi, Petersen, Ulha-Cintra, Sawaya, Chemical studies on the giant earthworm Glossoscolex giganteus (Leuckart) (1965) Comparative Biochemistry and Physiology, 16, pp. 491-496Haughton, Kerkut, Munday, The oxygen dissociation and alkaline denaturation of haemoglobins from two species of earthworm (1958) J. exp. Biol., 35, pp. 360-368Igarashi, Kimura, Kajita, Analysis of oxygen equilibria of the giant hemoglobin from the earthworm Eisenia foetida using the Adair Model (1986) J. Biochem., 109, pp. 256-261Meirelles, Oliveira, De Paula, Marangoni, Marques, Erythrocruorin of Glossoscolex paulistus (Oligochaeta, Glossoscolecidae): presence of disulfide bonds and their relation to ligand properties (1985) Comp. Biochem. Physiol., 82 B, pp. 203-205Meirelles, Oliveira, Oliveira, De Paula, Marangoni, Rennebeck, Erythro-cruorin of Glossoscolex paulistus (Oligochaeta, Glosso-scolecidae): dissociation at alkaline pH and its ligands properties as revealed by chemical, immunochemical and electron micoscopy studies (1987) Comp. Biochem. Physiol., 88 A, pp. 377-379Ochiai, Dissociation and oxygen equilibrium properties of the earthworm (Pheretima hilgendorfi) hemoglobin (1983) Arch. Biochem. Biophys., 226, pp. 111-117Ochiai, Dissociation and oxygen equilibrium properties of the extracellular hemoglobin of Eisenia foetida (1984) Arch. Biochem. Biophys., 231, pp. 136-143Perussi, Imasato, Tabak, Meirelles, Fluorescence study of the extracellular hemoglobin of Glossoscolex paulistus erythrocruorin (1990) Phys. Chem. Phys. Med. NMR, 22, pp. 159-168Perussi, Souza, Tinto, Imasato, Meirelles, Tabak, Oxidation of the extracellular hemoglobin of Glossoscolex paulistus (1990) Invertebrate Oxygen Carriers, , S. Vinogradov, O. Kapp, Springer, BerlinShortle, Meeker, Gerring, Effects of denaturants and low concentration on the reversible denaturation of Staphylococcal nuclease (1989) Arch. Biochem. Biophys., 272, pp. 103-113Silva, Villas-Boas, Bonafé, Meirelles, Anomalous pressure dissociation of large protein aggregates (1989) J. biol. Chem., 264, pp. 15863-15868Tsuneshige, Imai, Hori, Tyuma, Gotoh, Spectrophotometric, electron paramagnetic resonance and oxygen binding studies on the hemoglobin from the marine polychaete Perinereis aibuhitensis (Grube): comparative physiology of hemoglobin (1989) J. Biochem., 106, pp. 406-417Vinogradov, Shlom, Kapp, Frossard, The dissociation of annelid extracellular hemoglobins and their quaternary structure (1980) Comp. Biochem. Physiol., 67 B, pp. 1-16Weber, Functions on invertebrate hemoglobins with special reference to adaptation to environmental hypoxia (1980) Am. Zool., 20, pp. 79-101Weber, Cationic control of O2 affinity in lugworm erythrocruorin (1981) Nature, 292, pp. 386-38