Behavior Of Hemolymph Amebocytes From Planorbidae In The Presence Of Schistosoma Mansoni Larvae Parasitism, By Inoculation Of Indian Ink Or Fracture Of The Shell [comportamento Dos Amebócitos Circulantes De Moluscos Planorbídeos Frente Ao Parasitismo Por Larvas De Schistosoma Mansoni, Inoculação De Tinta Nanquim E Fratura Da Concha]

The behavior of the hemolymph amebocytes of Biomphalaria glabrata and Biomphalaria tenagophila was studied by means of the infection by the Schistosoma mansoni, using inoculation with Indian ink and fracture of the shell. A differencial count of the amebocytes in the hemolymph was made with special attention given to the types of cell found in each sample. Histopathological evaluation of the molluscs exposed to the Schistosoma mansoni miracidia and analyses of the amebocytes were made. Correlation between the variance of the number of hemolymph amebocytes and tissue reaction was also made. The morphologycal study of amebocytes was carried out with the help of a phase microscope. An increase in granulocytes in Biomphalaria tenagophila occured only when the molluscs were infected by Schistosoma mansoni, which suggests the specificity of the reaction to the parasitism. Comparing the results obtained with B. glabrata and B. tenagophila it is concluded that these molluscs show different amebocitary behavior in the presence of the diverse stimuli used.304332340Abdul-Salam, J.M., Michelson, E.H., Biomphalaria glabrata amebocytes: Effects of Schistosoma mansoni infection on in vitro phagocytosis (1980) J. Invertebr. Pathol., 35, pp. 241-248Amen, R.I., Baggen, J.M.C., Bezemer, P.D., Jong-Brink, M., Modulation of the activity of the internal defence system of the pond snail Lymnaea stagnalis by the avian Schistosoma trichobilharzia ocellata (1992) Parasitology, 104, pp. 33-40Bayne, C.J., Buckley, P.M., De Wan, P.C., Citotoxity of hemocytes from susceptible snail hosts for sporocysts in plasma from resistant Biomphalaria glabrata (1980) Exp. Parasitol., 50, pp. 409-416Cheng, T.C., Rodrick, G.E., Foley, D.A., Koehler, S.A., Release of lysosyme from hemolymph cells of Mercenaria mercenaria during phagocytosis (1975) J. Invertebr. Pathol., 25, pp. 261-265Cheng, T.C., Butler, M.S., Experimentally induced elevations in acid phosphatase activity in the hemolymph of Biomphalaria glabrata (Mollusca) (1979) J. Invertebr. Pathol., 34, pp. 119-129Granath, W.O., Yoshino, T.P., Schistosoma mansoni: Passive transfer of resistance by serum in the vector snail Biomphalaria glabrata (1984) Exp. Parasitol., 58, pp. 188-193Guaraldo, A.M.A., Magalhães, L.A., Rangel, H.A., Pareja, G., Evolução dos esporocistos de Schistosoma mansoni Sambon, 1907 em Biomphalaria glabrata (Say, 1818) e Biomphalaria tenagophila (D' Orbigny, 1835) (1981) Rev. Saúde Pública, 15, pp. 436-448Loker, E.S., Bayne, C.J., In vitro encounters between Schistosoma mansoni primary sporocysts and hem olymph components of susceptible and resistant strains of Biomphalaria glabrata (1982) Am. J. Trop. Med. Hyg., 31, pp. 999-1005Morona, D., Jourdane, J., Aeschlimann, A., Étude de l'évolution in vitro des granulocytes de l'hemolymphe de Biomphalaria glabrata (Gastropoda, Planorbidae). Étude au microscope electronique (1984) Ann. Parasitol. Hum. Comp., 59, pp. 467-475Paraense, W.L., Corrêa, L.R., Sobre a ocorrência de duas raças biológicas do Schistsosoma mansoni no Brasil (1963) Ciên. Cult., 15, pp. 245-246Ratcliff, N.A., Invertebrate immunity. A primer for the non-specialist (1985) Immunol. Letters, 10, pp. 253-270Santana, J.V., Magalhães, L.A., Rangel, H.A., Quantificação da atividade fagocitária de amebócitos de moluscos Biomphalaria glabrata livres de infecção e infectados por Schistosoma mamsoni (1985) Anais, , Congresso da Sociedade Brasileira de Medicina Tropical, 21o, São Paulo, 1985. São Paulo, C. Baliero Editera. Resume no 58Sminia, T., Van Der Knaap, W.P.W., Cells and molecules in molluscan immunology (1987) Dev. Comp. Immunol., 11, pp. 17-28Stumpf, J.L., Gilbertson, D.E., Hemocytes of Biomphalaria glabrata. Factor affecting variability (1978) J. Invertebr. Pathol., 32, pp. 177-181Wegge, L.E., The activity of amoebocytes and of alkaline phosphatase during the regeneration of wall of the snail Helix aspersa (1951) Q. J. Microsc. Sci., 92, pp. 307-321Wegge, L.E., Mittler, T., Shell recognition in some Brithish molluscs (1953) Nature, 171, pp. 528-52