Factorial Design Of Electrolyte Systems For The Separation Of Fatty Acids By Capillary Electrophoresis
- De Oliveira M.A.L.
- Micke G.A.
- Bruns R.E.
- Tavares M.F.M.
DOIAbstract
In this work, a capillary zone electrophoretic methodology using UV indirect detection (224 nm) for the analysis of fatty acids (FAs) in saponified oils is proposed. The electrolyte consisted of a 5 mmol l-1 phosphate buffer, pH 7, containing 4 mmol l-1 sodium dodecylbenzenesulfonate (SDBS) as chromophore, 4 mmol l-1 dimethyl-β-cyclodextrin and 45% acetonitrile (ACN). The composition of the electrolyte was optimized by a 23 factorial design with triplicate at the central point. The design established practical concentration boundaries for SDBS and ACN. In a defined concentration range of 2-4 mmol l-1, SDBS can certainly be used as a chromophore for indirect detection without imparting excessive baseline noise. For ACN, a suitable interval o...
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