Linear And Non-linear Micro-spectroscopy In An Optical Tweezers System

We developed a set up consisting of an Optical Tweezers plus linear and non-linear micro-spectroscopy system to add the capabilities of manipulation and analysing the captured object. For the confocal micro-spectrometer we used a 30 cm monochromator equipped with a cooled back illuminated CCD. The spectroscopic laser system included a cw and a femtosecond Ti:sapphire lasers that allowed us to perfom raman, hyper-raman, hyper-rayleigh and two-photon excited (TPE) luminescence in trapped particles with an Nd:YAG cw laser. With the cw Tiisapphire laser we obtained raman spectra of a single trapped polystyrene microsphere and red blood cells and silicon to evaluate the performance of our system. The femtosecond Ti: sapphire laser was used to observed hyper-rayleigh and hyper-raman peaks of SrTiO3 with 60s integration time only. 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