Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
- Nascimento, Elvira Maria Mendes
- Gehrke, Flávia de Sousa
- Maldonado, Rosa Amélia
- Colombo, Silvia
- Silva, Luiz Jacintho da
- Schumaker, Teresinha Tizu Sato
Publication date
November 2015
DOI Publisher
FapUNIFESP (SciELO) Journal
Memórias do Instituto Oswaldo Cruz Language
EnglishAbstract
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.27727
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