Molecular detection and phylogenetic analysis of Aujeszky's disease virus, parvovirus and porcine circovirus type 2 of swine in Montenegro

Primenom metode PCR u ispitanim uzorcima svinja ustanovljeno je prisustvo virusa Aujeckijeve bolesti, parvovirusa svinja i svinjskog cirkovirusa tip 2. Metodom direktnog sekvenciranja po Sanger-u određen je redosled nukleotida dela gB gena virusa Aujeckijeve bolesti. Tri nukleotidne sekvence virusa Aujeckijeve bolesti uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa sekvencama navedenog virusa izolovanim u u Mađarskoj, Grčkoj i SAD. Na osnovu rezultata filogenetske analize ustanovljeno je da sva tri virusa Aujeckijeve bolesti identifikovana kod svinja u Crnoj Gori pripadaju genotipu I navedenog virusa. Metodom direktnog sekvenciranja po Sanger-u određen je redosled pet odabranih parvovirusa svinja identifikovana u Crnoj Gori. Na osnovu rezultata filogenetske analize parvovirusi svinja identifikovani u Crnoj Gori grupisani su zajedno sa sojevima navedenog virusa poreklom Velike Britanije, Kine, Brazila i SAD. Dobijeni rezultati filogenetske analize su pokazali da svinjski parvovirusi poreklom od svinja sa teritorije Crne Gore pripadaju klasteru E navedenog virusa. Primenom metode direktnog sekvenciranja po Sanger-u određen je redosled nukleotida dela ORF1 regiona genoma virusa PCV2. Nukleotidne sekvence identifikovanih PCV2 virusa imale su visok stepen sličnosti sa sojevima navedenog virusa izolovanim u Italiji, Austriji, Srbiji, Nemačkoj i Kini. Dobijeni rezultati filogenetske analize šest identifikovanih virusa PCV2 su potvrdili da njih pet pripada genotipu PCV2b, a jedan genotipu PCV2c.The presence of Aujeszky's disease virus, porcine parvovirus and porcine circovirus type 2 were detected by PCR method. Nucleotide sequences of Aujeszky's disease virus identified in swine in Montenegro were determined by direct sequencing method according to Sanger. Three nucelotide sequences included in phylogenetic analysis were highly similar with nucleotide sequences of Aujeszky's disease virus strains identified in Hungary, Greece and USA. According to results of phylogenetic analyisis, it was concluded that all three viruses belongs to genotype I. Nucleotide sequences of five porcine parvoviruses identified in Montenegro were determined by direct sequencing method according to Sanger and were highly similar with nucleotide sequences of porcine parvovirus strains isolated in United Kingdom, China, Brasil and USA. Phylogenetic analysis of five porcine parvoviruses revealed that all of them belongs to cluster E. The nucleotide sequences of six porcine circovirus type 2 identified in Montenegro were determined by direct sequencing method according to Sanger and were highly similar with nucleotide sequences of PCV2 strains isolated in Italy, Austria, Serbia, Germany and China. The phylogenetic analysis of six porcine circovirus type 2 showed that five of them belongs to genotype PCV2b, and one to genotype PCV2c