Add to ORKGA direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis
The human Xp21.3-p22.1 region is poorly mapped relative to other X chromosome regions. To target cos...
Mouse A9 cells containing human chromosome 7 tagged with pSV2neo were irradiated with X-rays and fus...
This thesis describes the construction and analysis of a cosmid library specific for the human Y chr...
The ubiquitous nature of the Alu sequence throughout the human genome forms the basis of an assay we...
We describe the construction and characterization of a human X-chromosome-specific yeast artificial ...
We have constructed cosmid libraries from flow-sorted human chromosomes X and 21, each of which cont...
X-linked agammaglobulinaemia (XLA) was previously mapped using genetic linkage analysis to Xq22. No ...
We have employed an irradiation and fusion procedure to generate somatic cell hybrids containing var...
Three yeast artificial chromosome (YAC) libraries were constructed using two human cell lines and th...
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containin...
Summary. Non-isotopic in situ hybridization of chromo-some-specific alphoid DNA probes has become a ...
The original objective of the work described in this thesis was the development of genetic and physi...
The Alu-polymerase chain reaction (Alu-PCR) was applied to selectively amplify DNA sequences from hu...
Hybrid cells were obtained by virus-induced fusion of hamster cells with irradiated human cells. The...
A reciprocal probing method is described which uses pooled cDNA probes to order chromosome specific ...
The human Xp21.3-p22.1 region is poorly mapped relative to other X chromosome regions. To target cos...
Mouse A9 cells containing human chromosome 7 tagged with pSV2neo were irradiated with X-rays and fus...
This thesis describes the construction and analysis of a cosmid library specific for the human Y chr...
The ubiquitous nature of the Alu sequence throughout the human genome forms the basis of an assay we...
We describe the construction and characterization of a human X-chromosome-specific yeast artificial ...
We have constructed cosmid libraries from flow-sorted human chromosomes X and 21, each of which cont...
X-linked agammaglobulinaemia (XLA) was previously mapped using genetic linkage analysis to Xq22. No ...
We have employed an irradiation and fusion procedure to generate somatic cell hybrids containing var...
Three yeast artificial chromosome (YAC) libraries were constructed using two human cell lines and th...
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containin...
Summary. Non-isotopic in situ hybridization of chromo-some-specific alphoid DNA probes has become a ...
The original objective of the work described in this thesis was the development of genetic and physi...
The Alu-polymerase chain reaction (Alu-PCR) was applied to selectively amplify DNA sequences from hu...
Hybrid cells were obtained by virus-induced fusion of hamster cells with irradiated human cells. The...
A reciprocal probing method is described which uses pooled cDNA probes to order chromosome specific ...
The human Xp21.3-p22.1 region is poorly mapped relative to other X chromosome regions. To target cos...
Mouse A9 cells containing human chromosome 7 tagged with pSV2neo were irradiated with X-rays and fus...
This thesis describes the construction and analysis of a cosmid library specific for the human Y chr...