Microrheology with optical tweezers: Peaks and troughs

Since their first appearance in the 1970s, optical tweezers have been successfully exploited for a variety of applications throughout the natural sciences, revolutionising the field of microsensing. However, when adopted for microrheology studies, there exist some peaks and troughs on their modus operandi and data analysis that I wish to address and possibly iron out, providing a guide to future rheological studies from a microscopic perspective