A common proviral integration region, fit-1, in t-cell tumors induced by myc-containing feline leukemia viruses

Feline leukemia viruses carrying transduced v-myc genes (myc-FeLV) induce tumors of clonel origin, suggesting that activated myc alone is not sufficient for tumorgenesis. To investigate the hypothesis that insertional mutagenesis plays a role by activating genes which collaborate with v-myc, we looked for evidence of common proviral integration sites in these tumors. By inverse polymerase chain reaction we identified a 6-kb domain, designated fit-1, in which FeLV proviruses were inserted in four of nine (44%) T-cell tumors induced by myc-FeLV. The fit-1 locus was mapped to feline chromosome B2 and appears to be distinct from known oncogenes located on this chromosome. Fit-1 represents a novel common proviral integration region which may harbor a cellular gene which acts in concert with the myc gene in T-cell tumorigenesis