The Fat Protective Effect of Fish Oil, Sunflower Seed Oil and Corn Oil on Fluid Rumen Fermentation Parameters

ABSTRACT: This study was aimed to obtain oil and the exact saponification optimization to protect unsaturated fats, which does not interfere with the fermentation of rumen fluid. The studied the protective effects of unsaturated fat as sheep feed supplement on fermentation parameters of sheep rumen fluid in vitro. Three oil comparising lemuru fish oil (LFO), sunflower seed oil (SSO) and corn oil (CO) each made with a combination of saponification soap and capsulation. Manufacturing unsaturated fatty acids soap was protected by heating and stirring each oil and 20% NaOH solution (caustic soda), added with10% starch solution to form soft and elastic paste (gel). Ratio of oil volume ratio, 20% NaOH solution and starch was 1: 2: 1. Pasta was allowed to solidify within one night (12 hours), then sliced into thin slats with knife and soaked in saturated CaCl solution to harden the gel slats which was then crushed by squeezing to form small grains (crystals). Then dried (moisture content of 10 to 20%). Soap samples were taken for in vitro analysis to determine the parameters of the rumen fermentation conditions by observing parameters rumen fluid pH, VFA, NH2, and rumen microbial protein. The results showed that the three oils were not significantly affecting (P>0.05) pH, microbial proteins, VFA, and NH3, except for significantly low digestibility of dry matter (DM) and organic matter (OM) (P