Polymorphism of mtDNA Cytochrome b Gene of Local Cattle in Indonesia

The aim of this study was to evaluate of genetic diversity of local cattle in Indonesia. We analyzed the polymorphism of mtDNA cytochrome b (cytb) gene using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Seventy-three Indonesian local cattle were used in the study that consisted of Madura cattle from Madura island (n = 15), Madura cattle from Sapudi island (n = 23), Limousin x Madura from Madura island (n = 5), Bali cattle from Kupang (n = 12), Aceh cattle from Aceh (n = 5), Pesisir cattle (n = 4), Bali cattle from West Sumatera (n = 1), and Java cattle from Pacitan (n = 8). The 464-bp fragment of cytochrome b (cyt b) gene was amplified using primers: forward primer of L14735 and reverse primer of H15149. The PCR product was digested using HinfI and TaqI restriction enzyme to identify the polymorphism of mtDNA cytochrome b (cytb) gene. The result showed that there was two haplotypes (A and B). Aceh and Pesisir cattle have 100% haplotype B meanwhile Bali cattle (from Kupang and West Sumatera) and Limura cattle have 100% haplotype A. Whereas, Madura cattle from Madura and Sapudi island and Java cattle have two different haplotypes (A and B). There was mt-DNA cytochrome b polymorphism of local cattle in Indonesia based on PCRRFLP analysis method. Since the genetic variation in local cattle showed the differences in the specific area, it is necessary to control the cattle populations based on the species. Keywords: Polymorphism; genetic variation; cytochrome b gene; local cattle; Indonesia * Corresponding author. Tel.:+62