EFEK EKSTRAK ETANOLIK DAUN JAMBLANG (Syzygium cumini (L) skeels) TERHADAP FAKTOR VIRULENSI Streptococcus mutans ISOLAT DARI KARIES GIGI ANAK USIA PRASEKOLAH

Caries is a major dental problem in children. The main bacterial cause of dental caries is Streptococcus mutans. This bacterium has virulence properties involved in the formation of biofilm on the tooth surface. Oral condition of children is often exposed to food and beverages containing sucrose, can affect the virulence of S. mutans. Jamblang (Syzygium cumini) leaves are potentially as anticariogenic material due to its antibacterial effect. The purpose of this study was to investigate antibacterial potency and effect of ethanolic extract of Jamblang leaves against virulence factors of S. mutans isolates from children�s caries, that is water-insoluble glucan synthetizing, acid production, and adhesion of S. mutans to hydroxyapatite. Analysis of the active compounds of the extract was carried out using thin layer chromatography (TLC) and Liquid Chromatograph-Mass Spectrography (LC-MS). Samples of S. mutans were isolated from children�s carious deciduous molar teeth. The experiment of virulence factors of S. mutans was carried out by analysing the effect of the extract concentration on the water-insoluble glucan synthetizing, acid production, and adhesion of S. mutans to hydroxyapatite. The concentrations of extract tested were 22.5%, 20%, 15%, and distilled water as a negative control. Non-parametric Kruskal-Wallis test was used to analyse data of this study. The results of TLC and LC - MS analysis showed the presence of flavonoid, tanin, and terpenoid in the leaves. Effect of extract�s concentration on the growth, water insoluble glucan synthesizing, and adhesion of S. mutans to hydroxyapatite were significant different (p<0.05) among groups. The concentration of ethanolic extract of 22.5% gave the greatest effect on the growth, on water-insoluble glucan synthetizing and on adhesion of S. mutans compare to the other two concentrations . The effect of ethanolic extract of Jamblang leaves against pH culture of S. mutans based on time contact increased significantly (p<0.05) between momentary contact to a contact time of 1 hour, and between contact time of 1 hour to 8 hour. The effect of ethanolic extract of Jamblang leaves against pH culture of S. mutans based on the concentrations of the extract were significantly different (p