PENGARUH JUMLAH FOLIKEL PADA OVARIUM TERHADAP KUALITAS MORFOLOGI OOSIT SAPI YANG DIKOLEKSI SECARA IN VITRO

The quality of the oocyte morphology can be evaluated using direct and indirect methods. Direct method is observation oocyte quality by asseing directly observed object. Indirect method have the advantage to know the quality of the oocyte through the number of ovarian follicles. Oocyte quality has possibility determine the quality of embryo production both in vivo and in vitro. Thus, this study was conducted in order to determine the effect of the number of cattle�s ovarian follicles on morphological quality of oocytes in vitro. Ovaries were taken from Giwangan Slaughterhouse, washed with 0.9 % NaCl that supplemented with antibiotics ( 100 IU / ml penicillin G , 100 μ g / ml Streptomycin ) . The ovaries stored in a flask containing a solution similar to the washing solution at of 370C during the transportation to the laboratory . Oocytes were collected by aspiration using 10 ml syringe and 18 G needle that had been filled with 0.5-1 ml Phosphate Buffer Saline ( PBS ). The number of follicles were divided into 3 groups: group I ovary was the number of ovarian follicles 1-1