LARVICIDAL EFFECT OF CHLOROFORM EXTRACT FROM Annona muricata Linn FLOWERS AGAINST Aedes aegypti Larvae

Background: Dengue is the most common mosquito-borne viral disease. The main vector for Dengeu virus is the Aedes aegypti mosquito. Due to no vaccine for dengue till now, vector control is the main prevention for the spread of Dengue. Some parts of Annona muricata,a plant from the Annonaceae plant family have been reported to posses larvacidal activity against variety of larva. This research is specifically to find the larvacidal activity of the chloroform extract from Annona muricata flowers againt Aedes aegyti larva. Aims of study: The aims of the research is to develop larvacide against Aedes aegypti from Annona muricata flowers, to find the LC50 for the chloroform extract and to test whether increasing the dosage concentration will increase the larvae mortality. Methods: Third and fourth instar larvae of Ae. aegypti were introduced to varying concentration of chloroform extract from Sirsak (A. muricata) flowers for 24 hours under standardized condition. Results: The LC50 and LC90 value for the cloroform extract of A. muricata flowers after 24 hours of exposure are 1244,04 ppm and 3388,44 ppm respectively. Conclusion: Chloroform extract of A. muricata flowers have larvicidal activity against Ae. aegypti larvae but it is not effective. Mortality of the larvae increase with the increase in the chloroform extracts concentration