DETEKSI GEN mecA ISOLAT Staphylococcus aureus RESISTEN dan SENSITIF TERHADAP METISILIN ASAL SUSU SAPI PERAH

Staphylococcus aureus is the main cause for clinical and subclinical mastitis in dairy cattle. Until recently, mastitis has only been medicated with antibiotics, despite the fact that misuse of antibiotics can cause S. aureus resistance from antibiotics. Methicillin resistant staphylococcus aureus (MRSA) is one of the strains of S. aureus which is resistant to methicillin. MRSA was detected for the first time in cattle milk with mastitis and it was a pathogenic bacterium to humans. While mecA gene is responsible for the resistance from beta-lactam antibiotics such as methicillin, it was also reported to be found in methicillin sensitive S. aureus. The objective of this research is to detect the existence of mecA gene in methicillin resistant and sensitive S. aureus which are isolated from dairy cattle milk. MecA gene were detected from 32 methicillin resistant and sensitive S. aureus isolate from cattle milk from Boyolali, Pacitan, and Ponorogo. In those 32 S. aureus isolate, 28 are from subclinical mastitis cattle milk, 3 from normal cattle milk, and 1 from clinical mastitis milk. Reidentification of S. aureus was done by culture process on blood agar plate, gram stain, catalase test, coagulase test, mannitol fermentation ability with Mannitol Salt Agar (MSA), and voges proskauer test. Identification of S. aureus in a molecular level was done by detecting 23S rRNA gene using polymerase chain reaction (PCR). The determination of resistant and sensitive S. aureus by testing the bacterium on Mueller-Hinton Agar (MHA) using methicillin disc was done subsequently. PCR molecular detection was used to identify mecA gene as the target gene. PCR product of mecA gene was then sequenced to determine whether the amplified DNA fragment was actually mecA gene or not. The result of this research showed that all of the S. aureus isolates (100%) could be indentified conventionally as well as molecularly. 16 (50%) methicillin resistant and 16 (50%) methicillin sensitive were identified from the S. aureus isolates. MecA gene cannot be identified in all of the S. aureus isolates, both methicillin resistant and sensitive. 93.75% MRSA were found in the subclinical mastitis milk