AKTIVITAS ANTIOKSIDAN EKSTRAK KLOROFORM DAN METANOL DAUN Pandanus spp. DAN KORELASINYA DENGAN KARAKTER ANATOMIS

Pandanus is a genus that commonly grows in subtropic and tropic area like Indonesia. Daily activities, Pandanus are used as a dye, food aromas and crafts raw material. Pharmacologically, some species have been known as an antioxidant material. The research purposes was to find out the antioxidant activity of the seven species and its correlation with anatomical characters. The paramaters measured included the antioxidant activity, total phenolic content (TPC), total flavonoid content (TFC) and the anatomical characters. The sample extraction was conducted using maceration method with methanol and chloroform as the solvents. The antioxidant activity was measured using 1,1- dyphenyl,2-picrylhydrazyl (DPPH) method. Leaf anatomical characters wereprepared using free hand section method and observed under light microscope. The parameters measured were the thickness of leaf, cuticle, upper and lower epidermal, upper and lower hypodermal, palisade parenkim and sponge parenkim. Moreover the length of papillae and the amount of secretion cells were also measured.The data were analyzed using Anova two ways and LSD test with the corection levelα = 5%, After the anava analyzing, the correlation between antioxidant activity and anatomical datas were analyzed using regression correlation test. The results show that antioxidant activity of the seven species of Pandanus were obviously very low compared toquersetin. P. kurzii that was extracted with methanol had the highest capability to capture free radical with percentage of 37,18%. Moreover, the correlation among total phenolic and flavonoid contents, antioxidant activity and anatomical characters were analyzed using regression correlation test. The results show that total phenolic and flavonoid contents have positive correlation with antioxidant activity of methanolic extract. But, the compounds have no correlation with antioxidant activity of chloroformic extract. Positive correlation are also occur between the thickness of palisade and antioxidant activity of chloroformic extract. The last, there were positive correlation between the number of cell secretory and total phenolic content of methanolic extract, total flavonoid content of methanolic extract and total flavonoid content of chloroformic extract