VALIDASI METODE ANALISIS DAN PENETAPAN KADAR PIRIDOKSIN, NIKOTINAMID, TAURIN, DAN KAFEIN DALAM MINUMAN BERENERGI SECARA KROMATOGRAFI LAPIS TIPIS-DENSITOMETRI

Energy drink is classified as a food supplement which contains vitamins, minerals, and stimulants, such as caffeine and taurine. It is necessary to develop an analytical method that is simple, rapid, and efficient in order to evaluate the compounds in energy drinks. TLC-densitometry method was chosen to evaluate the pyridoxine, nicotinamide, caffeine and taurine in the energy drink sample. Taurine has only a very short chromophore group in its molecular structure and very weak absorption to the UV/Visible radiation. Therefore it is necessary for taurine to be reacted with ninhydrin reagent 10% to yield a purple compound (Ruhemann purple) as the derivatization method. The maximum wavelengths used in the detection of caffeine, nicotinamide, pyridoxine, and taurine after derivatization were 274 nm, 263 nm, 293 nm, and 489 nm, respectively. In this study, TLC plates of silica gel 60 F254 was used as the stationary phase and methanol : ethyl acetate: ammonia 25% (4 : 23 : 3) was used as the mobile phase. Analytical method validation was performed to determine the selectivity, linearity, limit of detection, limit of quantitation, precision, accuracy, and robustness of the TLC-densitometry method. Resolution values obtained from the nicotinamide, pyridoxine, and taurine were 0.996