DAYA ANTIBAKTERI KOMBINASI CHITOSAN-GENTAMISIN TERHADAP STAPHYLOCOCCUS EPIDERMIDIS DAN PSEUDOMONAS AERUGINOSA SECARA IN VITRO

Background: Staphylococcus epidermidis and Pseudomonas aeruginosa is a bacteria causing nosocomial infections that have experienced some kind of resistance to antibiotics, such as methicillin, penicillin, carbapanems, cephalosporin, and ticarcillin. The high rates of resistance would complicate the treatment of infections and increase the burden of the cost of treatment for the patient. Chitosan is known as polysaccharides that have unique properties, among others, have the effect of antibacterial, antifungal, biodegradable and good biocompatibility. Gentamicin is a bactericidal agent that is potent against several gram-positive bacteria and as a primary option in the treatment of gram-negative bacterial infections, often used in combination with other antibiotics. Current widespread use of antibacterials have increased antibiotic resistance, so that the antibacterial efficacy began to decline. In addition to the development of vaccines and antimicrobial agents has not kept pace with the increase in resistance. Therefore, the limited availability of antibiotics as well as the necessary time and cost in the discovery of new antibiotics, and therefore needed another method by combining existing antibiotics such as gentamicin with antimicrobial potential alternative as chitosan. Objective: Knowing the antibacterial combination of chitosan and gentamicin against planktonic cell growth of Staphylococcus epidermidis and Pseudomonas aeruginosa and the effect of the resulting combination. Method: This research was a descriptive study experimentally. Subjects were material test compound chitosan and gentamicin as well as test bacteria Staphylococcus epidermidis and Pseudomonas aeruginosa microbiology laboratory collection of the Faculty. Minimum inhibitory concentration (MIC) combination of chitosan and gentamicin against planktonic cells of Pseudomonas aeruginosa and Staphylococcus epidermidis examined using in vitro antimicrobial susceptibility test. While the Fractional Inhibitory concentration (KHF) chitosan and gentamicin against planktonic cells of Pseudomonas aeruginosa and Staphylococcus epidermidis examined using Checkerboard Methods. Result: Minimum inhibitory concentration (MIC) of chitosan in combination, against isolates S. epidermidis and P. aeruginosa in a variety of different strains show results, S. epidermidis 64 has a combination KHM 1.56 ug / ml, S. epidermidis had MIC combination 236 0.78 ug / ml, and S. epidermidis had MIC combination 134 1.56 ug / ml. While P. 958 has a combination aeruginosa MIC of 6.25 ug / ml and P. aeruginosa has an MIC kombinasi25 1105 ug / ml. While the minimum inhibitory concentration (MIC) of gentamicin in combination, against isolates S. epidermidis and P. aeruginosa in a variety of different strains show results, S. epidermidis had MIC 64 combination 0, L25 ug / ml, S. 236 epidermidis had MIC combination of 0.05 mg / ml, and S. epidermidis had MIC combination 134 0.125 ug / ml. While P. 958 has a combination aeruginosa MIC 0.25 mg / ml and P. aeruginosa 1105 has an MIC of combination 0.125 mg / ml. Conclusion: Antibacterial combination of chitosan and gentamicin to inhibit the growth of bacteria S. epidermidis and P. aeruginosa