KARAKTERISASI DAN IDENTIFIKASI BAKTERI HALOFILIK PENGHASIL PROTEASE DARI BLEDUG KUWU

Halophilic bacteria are group of bacteria that live and growth in high salt concentration environment. It could be found in sea water, pond, and mud at which the salt concenration is high. Among members of halophilic bacteria, some of them could produce extracellular protease. Protease useful enzyme for various industries such as tannery, food, and detergent industries. On our previous study, we obtained four halophilic bacteria which were able to produce protease, i.e. BKL-1, BKL-4, BKL-5 and LC-2. The protease produced by those isolates has been biochemically characterized. The objective of this research was to characterize and identify those isolated halophilic bacteria. Characterization was examined based on salinity tolerance and temperature tolerance. From the observation we concluded those isolates were moderate halophilic and mesophilic bacteria. Identification was carried out based on the morphological observation, biochemical test, and 16S rRNA sequences analysis. The results concluded that BKL-1 and LC-2 were determined to be Staphylococcus arlettae, BKL-4 was Halomonas smyrnensis, and BKL-5 was Halomonas sp