PENGARUH TOPIKAL ZINC TERHADAP JUMLAH FIBROBLAS PADA HARI PERTAMA DAN KETIGA PASCA INCISI KULIT TIKUS Sprague Dawley YANG DIBERI PAKAN LEMAK TINGGI

The obesity is one of the wound healing phase inhibitor factor. Zinc was reported able to accelerate the wound healing by topical application. This study was aimed to determine the effect of zinc to the number of fibroblasts during wound healing phase in Sprague Dawley rat given high-fat diet . Thirty two rat were used in this study. The rat were allotted into four groups (A, B, C, and D) of four. Group A and B were given normal feed, while group C and D were given high-fat diet. Rats were anaesthetized and a skin) incision was made in the right dorsal part aseptically, then it approximattly 2cm. After surgery, groups A and C were given vaseline ointment on the wound, while groups B and D were given zinc 10% ointment every day. At the first day four rats werw taken from each group for biopsy. Then skin tissue was fixed in neutral buffered formaline(NBF) 10 % for histopathology preparation with haematoxylin-eosin (HE) staining. At the third day, the same treatment performed on the remaining rats. Fibroblasts calculation was performed on six fields of view around the incision area with 100x magnification. The results showed that the number of fibroblasts at third day was significantly higher (P