ISOLASI, IDENTIFIKASI DAN UJI SENSITIVITAS BAKTERI Staphylococcus aureus DARI SUSU SAPI PENDERITA MASTITIS UBKLINIS DI SABRANG WETAN DESA WUKIRSARI CANGKRINGAN TERHADAP ANTIBIOTIKA PENICILLIN G DAN TETRACYCLINE

Mastitis is one of diseases in dairy cows that can cause a loss for a breeder, especially decreasing number of production and milk quality. Inappropriate antibiotics application may cause bacterial resistance. The research is aimed to detect sensitivity of Staphylococcus aureus towards penicillin G and tetracycline. Sixteen samples of cow�s milk were cultured on Blood Agar Plate (BP) and Mac Conkey Agar (MCA) was then incubated for 24 hours at 37ºC. Coloni that grow were identified on Gram staining, catalase test, coagulase test and sugar fermentation test. To determine the sensitivity to the antibiotic penicillin G and Tetracycline bacteria that have been cultured on media BHI (Brain Heart Infussion) then cultured average detail in MHA (Muller Hinton Agar) and incubated at 37ºC for 24 hours with upend position. Resistant zone formed was measured with rule. The result was matched with interpreting tables standardized resistant zone Kirby-Baue to determine S. aureus sensitivity towards the antibiotic penicillin G and Tetracycline. The result of isolation shows that from 15 samples tested, 2 samples are positively infected by S. aureus. Test result of Staphylococcus sensitivity towards Penicillin G and Tetracycline shows that 2 samples of S. aureus are positively sensitive towards Penicillin G and Tetracycline. According to sensitivity test in this research, the researcher suggests to use Penicillin G and Tetracycline in subclinical mastitis treatment caused by Staphylococcus aureus