PENGARUH EKSTRAK TERPURIFIKASI HERBA SAMBILOTO (Andrographis paniculata (Burm.F.)Ness.) TERHADAP KADAR GLUKOSA DARAH DAN HISTOLOGI PANKREAS TIKUS DIABETES MELLITUS TIPE 2 DEFISIENSI INSULIN

Indonesia is a country of people with DM fourth largest after India, China, and America. Therefore we need an effective diabetes medicine primarily from natural materials because of the tendency for people who want to get back to nature. One herb that is often used for the treatment of the general public is Sambiloto. Sambiloto contain of chemical constituents which one of them a diterpene lactone, andrographolide. The purpose of this research was to find out the effects of purified extract of sambiloto on blood glucose levels and histopathology of the pancreas in test animals Wistar rats. Purified extract of Sambiloto was extracted by maceration using ethanol 90% and purified with n-hexane, identified by Thin Layer Chromatography and densitometry. Albino rats (at the age 2-5 day) were given with STZ i.p., then at the age of 3 months the diabetic rats insulin deficiency were divided into 4 groups (each group consisted of 6 rats): purified extract of Sambiloto at a dose of 343.6 mg/kg, dose of 1303.8 mg/kg, Glibenclamide (positive control) and CMC-Na 0.5% (negative control). Serum was taken and analyzed preprandial and postprandial blood glucose levels, then analyzed statistically. The pancreatic tissue of rats was preparated for the observed histologically for qualitative observations with Hematoxylin-eosin staining, the number of pancreatic beta cells quantitatively and the Immuno-Histochemistry. Andrographolide levels obtained at 16.09% in purified extract of Sambiloto The analysis showed no reduce in blood glucose levels significantly compared with the negative control. There were not improvements in pancreatic beta cells qualitatively, but increase in the number of pancreatic beta cells and an increase in expression of insulin, compared with the negative control group