JUMLAH SPERMATOSIT PRIMER DAN KADAR TESTOSTERON INTRATESTIKULER PADA TIKUS Sprague Dawley YANG DIINDUKSI STREPTOZOTOCIN DENGAN LATIHAN TERATUR TERUKUR

Background :Hyperglycemia often happens in people with diabetesmellitus. It affects on the increase of oxidative stress and the formation of advanceglycationend products. Both have an important role in the chronic complication on the reproductive organs : the testicle. Follicle Stimulating Hormone will stimulate Sertolicells for the spermatogenesis process, while Luteinizing Hormone will stimulate Leydig cells to produce testosterone hormone for the process of spermatogenesis. Growth Hormone/Insulin-like Growth Factor-1 is anautocrin/paracrinfactor in regulating the number and function of the mature Leydig cells. Growth Hormone/Insulin � like Growth Factor-1 serves as the proliferation stimulator and directmitogenic factor in stimulating the germcells proliferation. Objectives :This research is to study the effects of measured regular-exercise in changing the number of primary spermatocyte and the level of intratesticular testosterone in the testicles of Sprague dawleyrats induced by streptozocin. The research counts the number of primaryspermatocyte, the level of intratesticular testosterone and the correlation between the number of primary spermatocyte and the level of intratesticular testosterone. In detail, this also measures the germ cell layers and the diameter of seminiferous tubules. Methods :This research is a quash experimentwith a control group design on post test only. The subjects are 15 Sprague dawleyrats divided randomly into 3 groups. First group is a normal one. They do not get any streptozocin inductions and measured regular-exercise treatment. The second or the control group receives streptozocin inductions of 35 mg/kg body weight but they do not get any measured regular-exercise treatment. The third or the treatment group gets streptozocin inductions of 35 mg/kg body weight and measured regular-exercise treatment for 9 weeks. During the first 5 weeks, the intensity of the exercise is increased from 5 m/minute for 10 minutes to 17m/minute for 45 minutes. In the last 4 weeks, this group gets a constant intensity of the exercise that is 20m/minute for 60 minutes. After the exercise ends/ terminates, 1 testis is made for the preparat preparation for testicle histology by fitting staining and the other is homogenized to check its intratesticular testosterone level using ELISA method. The statistics is evaluated by One-way ANOVA test and multiple comparison of LSD Test. Results :The number of primary spermatocyte in the treatment group is significantly larger than the control group (p < 0.05). The germ cell layers, the diameter of seminiferous tubules and testosterone intratesticularlevels in this group are not larger significantly than the control group.The number of primary spermatocyte is correlated insignificantly to the intratesticular testosterone levels. (p = 0.4