ISOLASI DAN KARAKTERISASI ACTINOMYCETESASOSIASI SPONS PENGHASIL ANTIBIOTIK KOLEKSI PULAU BARRANG LOMPO MAKASSAR

Actinomycetes are very important microorganisms as bioactive secondary metabolites producers. Active substances of microba origin have been searched through a process of screening methods to obtain antibacterial compounds. The purposes of this research was to isolate and identify bacteria actinomycetes associated sponge from Barranglompo Island Makassar and to isolate, identify the antibacterial compounds from actinomycetes associated sponge by spectroscopic methode. Isolation of Actinomycetes from the sponge was done by spread plate method using SNA medium (Starch Nitrate Agar). Identification of actinomycetes bacteria was based on its morphology and physiology, and molecular characters with gen16S rRNA. Non-ribosome Peptide Sintetase (NRPS) gene detection to determined the correlation of the antibacterial compound produced. The most active actinomycetes isolates fermented and extracted with ethyl acetate. The antibacterial compound was separated using vakum liquid chromatography and preparative Thin Layer Chromatography (TLC). The structure determination was done on the basis of spectroscopy data (UV-Vis spectral data, IR, 1H-NMR, 13C- NMR, 2D NMR, and mass spectra). Molecular characterization showed that the isolates of actinomycetes closed related to Streptomyces parvulus and Uncultured Streptomyces sp. with 81.68% similarity. The results obtained from the characterization of antibacterial compounds based on existing data suggest that these compounds suspected Actinomycin D or Dactinomycin. This conclusion is supported by the NRPS gene sequencing results that show a similarity with the S. parvulus known to produce secondary metabolites of a peptide compound Actinomycin D (Dactinomycin). This compound displayed minimum inhibitory concentration 0.0195 µg / ml against Staphylococcus aureus resistant to antibiotic