UJI KERENTANAN INSEKTISIDA ORGANOFOSFAT DAN DETEKSI TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA Aedes aegypti DI KOTA KENDARI

Background: Dengue Haemoragic Fever disease which caused by dengue virus and spread by both Aedes aegypti and Aedes albopictus. Vectors were controlled through organofosfat insecticide usage (melathion and temefos), although the fogging with inseticide focused have been applied and followed by abatisasi selective, dangue virus circulation in Aedes aegypti might be still occured because the presistence of Aedes aegypti were spread through transovarial where the frequency increased filial (F)-7. Objectives: To proof susceptible status on Aedes aegypti towards organofosfat insecticide and its potency to infect dangue virus through transovarial at Kendari. Method: Design of the study was cross-sectional. Population of the study was larva and Aedes aegypti from F1�s egg generation. Biochemist and biological test are done to detect the suspectability status of Ae aegypti towards organophospate insecticide, whereas immunohistochemist on head squash means was done towards 1 week adult female Ae. Aegypti which has never been sucking the blood to figure out the transmission transovarial index of dengue virus. Result: Aedes aegypti larva with biochemist test at high and low endemic areas qualitatively showed the average of resistence status towords organofosfat insecticide, however high endemic area showed a higher precentage than low endemic area. Bioessay test on Aedes aegypti larva towards temefos for both endemic areas showed equal on the resistance status, and those areas also showed equal on susceptible status towards malathion. Transovarial Transmission Indeks (TTI) at high endemic area was higher 26 % than at low endemic area was 12 %. Conclusion: The percentage of Aedes aegypti Larva which resists towords organofosfat insectiside at high endemic area (Kelurahan Kadia) was higher meaningful than at low endemic area (Kelurahan Kambu) temefos had been resistence, Malathion is still being suspectible. Transovarial Transmission Indeks (TTI) of Aedes aegepty towards Dengue virus at high endemic area was higher meaningfull than at low endemic area