PURIFIKASI ANTRAKINON DAN MUSILAGO EKSTRAK DAUN SENNA (Cassia angustifolia Vahl) SERTA UJI EFEK KOMBINASINYA PADA AKTIVITAS LAKSATIF

Senna leaf is well known for many years ago by all people as a laxative because the content of its anthraquinone, with levels of 1.5 to 3%. Furthermore, it also contains of mucilage 10%. Laxative effect of anthraquinone or bulk laxative effect of mucilage possibility can combine to facilitate the defecation. The purpose of this study is to determine the purification method that produces fractions with high levels of mucilage and anthraquinone and also their laxative activity in mice. Purification method is done by three ways, namely A: consist of ethanol extract 96%, and C from ethanol extract 50% (EE50) which are fractionated with ethanol 96%. Each anthraquinone fraction levels set by uv-vis spectrophotometer (calculated on sennosida A), while the level of mucilage fraction determined gravimetrically. The combination effect is preceded by study of laxative potency in crude extracts, continued with anthraquinone and mucilage fractions of the most active extracts. Mice were divided in 13 groups, group 1-4 as negative control (aquadest), vehicle control (CMC Na), positive control (bisacodil and lactulose), 5-11 treated with micellanous senna leaves extract 420, 2100, and 4200 mg/kgbw, and 12-13 treated with fraction of anthraquinone and mucilage. The laxative potency seen from the defecation patterns with parameters accumulation of wet weight stool every 8 hours and total of 24 hours, stool water content in AUC2-2, and the frequency of occurrence of diarrhea. Data were analyzed with the statistical convidence level of 95%. Test results indicates that the highest levels of anthraquinone obtained from methods A and the highest levels of mucilage obtained from method C. Extract as the relatively polar (water extract) dosage 2100 mg/kgbb is relatively higher in laxative activity than semi-polar extracts or nonpolar extracts at the same dosage. Anthraquninone and mucilage fractions of water extract do not affect the defecation patterns of mice