PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS : PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS PADA AYAM BROILER

The experiments were conducted to determine some properties of the indigenous lactic acid bacteria (LAB) probiotics and its influence on biological expressions of broiler chickens. The LAB probiotics consisted of Lactobacillus murinus (Ar3), Streptococcus thermophilus (Kp2) and Pediococcus acidilactici (Kd6) were assessed on their ability to adhere to the chicken ileal epithelial cells (IEC) in vitro and in vivo as well. In the first stage of the experiments were reared 30 broiler chickens as the collecting source of the IEC preparation to the adhesion assay (in vitro) and 48 chickens were reared for sampling examination of SEM (in vivo). In the second stage, the 60 broiler chickens were assigned randomly into 4 treatment groups of LAB probiotics (mixed culture) orally supplementation consisted of (T0) one group of unsupplemented and three groups of supplemented chickens as much as 107 (T1), 108 (T2) and 109CFU/ml/bird/day (T3) respectively. All of treatment groups study the biological expression consisted of productivity performance such as feed consumption, body weight gain, feed conversion ratio and the intestinal histology structure such as villus height, villus width and crypt depth (μm) in duodenum, jejunum and ileum. The third stage used 90 chickens for studying the competitive exclusion of Salmonella pullorum by LAB probiotic strains. The broiler diet was formulated to meet the NRC recommendations and were provided ad libitum. The data were analyzed using analysis of variance followed by Duncan New Multiple Range Test. The results of the experiment in Part 1 showed that LAB probiotic cells had a good ability to adhere to IEC as revealed by phase - contrast microscopy. The scanning electron micrographs showed a clear appearance of Lactobacillus murinus (Ar3), Streptococcus thermophilus (Kp2) and Pediococcus acidilactici (Kd6) attached firmly to the intestine of the supplemented broiler but there were none on the intestine of unsupplemented chicken. In Part 2, feed consumption, body weight gain and feed conversion ratio were affected significantly (P