PENGARUH MARMIN DARI Aegle marmelos Corr. TERHADAP KONTRAKSI OTOT POLOS TRAKEA MARMUT TERISOLASI YANG DIINDUKSI SECARA IMUNOLOGIS DAN NONIMUNOLOGIS

Marmin is a coumarin derivative compound that was isolated from the stem bark and root of plants Maja (Aegle marmelos Corr.). Marmin reported to have spasmolytic effect on the guinea pigs ileum contracted by either histamine or acetylcholine. Marmin also reported able to inhibit histamine release from RBL-2H3 cell cultures. Based on the facts, marmin have the opportunity to be developed as a drug for allergy. This research was conducted to determine the effect of marmin on guinea pig tracheal smooth muscle contraction induced by both endogenous or exogenous histamine, and also the effects of marmin in smooth muscle relaxation. Ovalbumin (3 µg/mL) was used to induce guinea pig tracheal smooth muscle contraction immunologically, whereas compound 48/80 (2.5 to 750 µg/mL) were used to induce contractions non-immunologically. In this study, the influence of marmin was tested in guinea pig tracheal smooth muscle contraction that induced by series concentration of histamine (10 -8 -10 -4 M). In addition, test relaxation marmin (5-100 μM) on isolated guinea pig trachea was also conducted. The results showed that marmin 100 μM able to inhibit contractions of isolated guinea pig tracheal smooth muscle induced non-immunologically by compound 48/80. The average of inhibitory percentage of marmin on tracheal contraction induced by compound 48/80 750 µg/mL was 90.95±2.82%. Marmin (10 and 100 μM) was also able to inhibit guinea pig tracheal smooth muscle contraction induced immunologically by ovalbumin. The average of inhibitory percentage of marmin 10 μM and 100 μM marmin were 25.86±7.57% and 43.49±9.24%, respectively. Marmin 100 μM was also able to shift pD2 histamine from 6.02±0.05 to 4.94±0.07. In addition, marmin was able to relax guinea pig tracheal smooth muscle and have a pD2 relaxation of marmin of 4.42 ± 0.02