HUBUNGAN ANTARA EKSPRESI VEGF DAN KEPADATAN MIKROVASKULAR DENGAN STADIUM, DERAJAT HISTOPATOLOGIS, DAN KETAHANAN HIDUP PADA PENDERITA KARSINOMA PAYUDARA DENGAN ER POSITIF DAN ER NEGATIF

Background: Regulation of receptor estrogen (ER) on vascular endothelial growth factor (VEGF) have a role on angiogenesis of breast carcinoma, which the role of complex estrogen and ER induce angiogenesis by influencing VEGF and microvascular density (MVD). In breast cancer, ER and angiogenesis determine its therapy and prognosis. The role of angiogenesis in ER positive and negative of breast cancer and their correlation with tumor stage, histopathological grade, and survival were not yet known. betwen the expression Aim: To investigate the correlation of VEGF and MVD with tumor stage, histopathological grade, and survival of breast cancer patient with ER positive and negative. Design Method: of the study was cross sectional with retrospective data, consists of 50 cases of invasive duct carcinoma of the breast from Sardjito Hospital. Tumor stage was established based on tumor size, lymph status, and metastasis. Histopathological grade was classified into well, moderate, and poor. Survival was determined from first diagnosis (2003) until the end of study (December 2010). The specimens were stained immunohystochemically with monoclonal antibody against ER, VEGF, and vWF. ER status was classified as ER positive and negative. Tumor with ER positive staining more than 10% was judged as ER positive. The expression of VEGF was counted based on the number of tumor cells which cytoplasm expressed VEGF/100 cancer cells in five high power fields. MVD was determined from the number of microvessels which endhotelial exspressed vWF in five hot spot areas. The correlation among its variable will be analyzed with Spearman and Chi-Square test, and the survival will be analyzed with logistic regression test and Kaplan-Maier method. Among breast cancer ER positive and negative t Result: here were: no correlation between VEGF with tumor size (p:0.789 and p:0.254), lymph node status (p:0.643 and p:0.904), metastasis (p:0.124 and p:0.327), and histopathological grade (p:0.202 and p:0.225). There were no correlation between MVD with tumor size (p:0.849 and p:0.967), lymph node status (p:0.854 and p:0.888), metastasis (p:0.095 and p:0.897), and histopathological grade (p:0.814 and p:0.750) on ER positive and negative. There were no correlation between VEGF (p:0.268 and p:0.196) and MVD (p:0.570 and p:0.934) with survival of ER positive / negative of breast cancer. VEGF expression have a correlation with tumor metastasis (p:0.031) while lymph node status have a correlation with survival breast cancer (p:0.027). The e Conclusion: xpression of VEGF and MVD have no correlation with several clinicopathologic parameter of breast cancer with ER positive and ER negative, but the expression of VEGF have a correlation with metastasis, while lymph node status have a correlation with survival