POLIMORFISME GEN GROWTH HORMONE (GH) PADA SAPI MADURA DAN PERSILANGAN LIMOUSIN-MADURA

The present study was conducted to identify polymorphism of growth hormone (GH) gene in Madura and Madura crossed (Limousin-Madura) cattle, and its association with growth traits. Blood samples of 58 cattle were taken from population of Madura and Madura crossed, then all cattle are located in the same area in Madura island. A 211 bp fragment of GH gene (spanning from the forth intron region to fifth exon) was amplified using GHF (forward) primer: 5�GCTGCTCCTGAGGGCCCTTC-3�, and GHR (reverse): 5�CATGACCCTCAGGTACGTCTCCG-3�. Polymorphism was identified with AluI restriction enzyme. The result indicated that two genotypes LL and LV were found at the GH gene in population of Madura crossed cattle. The frequencies of L and V alleles were 0.64 and 0.04, respectively. Between Madura and Madura crossed cattle had a significantly differences in their body weight estimation, chest girth, withers height, and body length. The appearance of LV genotype in Limousin-Madura had no associated with body weight estimation also several body measurement yet