UJI HISTOKOMPATIBILITAS BAHAN SUBSTITUSI TULANG GIPSUM DAN KOMBINASI GIPSUM DENGAN KARBONAT APATIT PADA JARINGAN LUNAK (Kajian In Vivo pada Sub Kutan Tikus)

Background. Combination of gypsum and carbonated hydroxyapatite has been shown to improve the bone healing process since gypsum that has been applied in many clinical practice may fill irregular and small defect and evoke minimal inflammatory response while carbonated hydroxyapatite has excellent osteoconductivity and good resorbtion rate. Soft tissue biocompatibility test is required prior to clinical application of bone substitutes since the material also contact to the soft tissue upon application and soft tissue response are more severe than the bone tissue response. Objective. The aim of this study was to evaluate histocompatibility of gypsum compare to combination gypsum with carbonated hydroxyapatite in subcutaneous tissue. Materials and Methods. Disk of gypsum and gypsum with carbonated hydroxyapatite were made with diameter of 6 mm and 0.8 mm thick. Twenty five healthy 3.5 � 4 month male Wistar rats were used. Each rat received one disk of gypsum and one disk of gypsum with carbonated hydroxyapatite in paravertebral subcutaneous tissue. Five rats were sacrificed each after 6 h, 5, 7, 14, and 21 days. 10 um paraffin sections of the implant and the tissue surround it were stained with Hematoxylin Eosin. Histological evaluation and scoring with a histological grading scale for soft-tissue implants were performed. A two-way ANOVA were used to analyze the results of the histomorphometrical analysis. The level of significance was set at P<0.05. Results and discussions. Acute inflammatory cells were found in 6 h, 5 and 7 d groups at similar number in both material. Chronic inflammatory cells such as foreign giant cells and lymphocytes as well as capsule were observed in day 7,14,21. There are no significant different (p>0.05) of histomorphometrics score between gypsum and gypsum with carbonated hydroxyapatite in each implantation periods (7, 14, and 21 days). Conclutions. Gypsum and combination between gypsum with carbonated hydroxyapatit e have the same soft tissue histocompatibility leve