The Investigation of Genetic Variation at Micro Satellite Loci in Mazandran Native Chickens

A total of 90 genomic DNAs were isolated from Mazendaran native chicken, through optimized and modified salting-out procedure. The samples were used in polymerase chain reaction (PCR) with 20 micro satellite markers. Amplified PCR- products with the markers were separated on a %8 Polyacrylamid gel. One locus (MCW216) was monomorphic. According to allele frequencies of 20 micro satellite sites, mean heterozgosity (H) and polymorphism information content (PIC) were calculated. The number of alleles varying from 1 to 6 and an estimate of average heterozygosity excluding the monomorphic data was calculated as 0.5872. The average heterozygosity and PIC value calculated from data on polymorphic and monomrphic loci was 0.5579 and 0.4939, respectively. Diversity estimates in this study are lower than the observed frequencies of heterozgotes reported in other species using micro satellite markers. Other parameters for intrapopulation variation and Hardy -Weinberg proportions were also considered. 14 of the micro satellite loci deviated from Hardy- Weinberg equilibrium. Some of micro satellite sites we re highly polymorphic, so they were effective markers for genetic diversity analysis. These results could provide basic molecular data for the research on the germplasm characteristics of Mazandaran native chicken