EKSTRAK ETANOLIK DAUN AWAR-AWAR (Ficus septicQ Burm. f.) MEMACU APOPTOSIS SEL KANKER PAYUDARA MCF-7 MELALUI PENEKANAN EKSPRESI Bcl-2

Alkaloid fenantroindolisidin memiliki aktivitas sitotoksik terhadap beberapa sel kanker. Ekstrak etanolik daun Awar-awar terbukti mampu meningkatkan aktivitas sitotoksik agen kemoterapi doxorubicin pada sel MCF-7.Penelitian ini bertujuan untuk mengetahui efek ekstrak etanolik daun Awar-awar (Ficus septica Burm. tJ terhadap pemacuan apoptosis sel kanker payudara MCF-7. Serbuk daun Awar-awar diekstraksi dengan cara maserasi menggunakan etanol 70% kemudian dipekatkan untuk memperoleh ekstrak etanolik daun Awar-awar. Penelusuran mekanisme molekuler efek sitotoksik ekstrak etanolik daun Awar-awar dilakukan dengan pengamatan apoptosis dengan metode double staining dan ekspresi protein Bcl-2 dengan metode imunositokimia. Pengamatan apoptosis dan ekspresi protein Bcl-2 pada aplikasi tunggal ekstrak daun Awar-awar menunjukkan kemampuan ekstrak menginduksi apoptosis dan menurunkan ekspresi protein Bcl-2. Hasil penelitian membuktikan bahwa ekstrak etanolik daun Awar-awar berpotensi untuk sebagai agen ko-kemoterapi. Alkaloid fenantroindolisidin showed cytotoxic activity against several cancer cells. Ethanolic leaf extract of Awar-awar proven to increase the cytotoxic activity of chemotherapy agent doxorubicin in MCF-7 cells. This study aims to determine the effect of Ethanolic leaf extract of Awar-awar in apoptosis induction of breast cancer cells MCF-7.Awar-awar leafpowder was extracted by maceration using 70% ethanol and then concentrated to obtain concentrated of Awar-awar. Apoptosis induction was examined by double staining method and the expression of Bcl-2 protein was observed by immunohistochemistry method. Single treatment of Awar-awar leaf extract showed the ability of extracts to induce apoptosis and downregulated the expression of Bcl-2 protein. Ethanolic leaf extract of Awar-awar is potential as co-chemoth~rapeutic agen