BENZOPHENONE GLUCOSIDE ISOLATED FROM THE ETHYL ACETATE EXTRACT OF THE BARK OF MAHKOTA DEWA [Pha/eria macrocarpa (Scheff.) Boerl.) AND ITS INHIBITORY ACTIVITY ON LEUKEMIA L1210 CELL LINE

ABSTRACT Isolation and elucidation of benzophenone glucoside from ethyl acetate extract of Phaleria macrocarpa bark and its inhibitory activity test against leukemia L 1210 cel/line have been done. The Phalaria macrocarpa bark were maceratedusing n-hexane,ethyl acetate, and ethanol,respectively.The ethyl acetate extract was then chromatographedon silica gel column and gradientlyeluted by n-hexane - ethyl acetate - ethanol with the composition from 20:1:0 until 0:0:1, gave eight fractions. Separation of fraction 6 using semipreparative HPLC on reverse phase column (Capeel/ Pak C-18 SG120, 15 mm I.D. x 250 mm) using methanol- water (40:60, 5 mUmin) gave a brown powder, with the melting point of 182.3 "C. Spectroscopic analysis and comparison of its physicochemical data, this compound was clarified as 2,4'-dihydroxy-4-methoxy~nzophenone-6-0-P.D-glucopyranoside (3). Inhibitory activity of its compound against leukemia L1210 cel/line showed that this compound exhibited inhibitory activity with IC50 was 5.1pg/mL