Identifikasi Ras Fisiologis Fusarium Oxysporum F.Sp. Cubense Berdasarkan Sifat Kompatibel Secara Vegetatif Dan Pembentukan Bahan Volatil : Characterization Of Physiological Races Of Fusarium Oxysporum F.Sp. Cubense...

Abstract: Race characterization of F. oxysporum Schlecht. fsp. cubense (E.F. Smith) Snyd. & Hans. by determining disease reaction is difficult because the result may be biased due to the variability of growing condition. This study is aimed to identify physiological races of F. oxysporum fsp. cubense in banana plantation in theprovince of Daerah lstimewa Yogyakarta by examining the relationship among the fungal pathogen isolates. The identification of physiological races of F. oxysporum fsp. cubense was based on vegetative compatibility groups (VCGs). The research was conducted in The Laborato of lifycology Faculty of Agriculture GMU on March to November 2000. Observation of heterokarion formed by the mutant of F. oxysporum fsp., cubense on selective medium was carried out in order to identify the compatibility of two different fungal isolates. Nitrate non utilizing (nit) mutants obtained without mutagen were used as the label. Nit mutant obtained from the same wild type isolates could form heterokarion on minimal agar medium containing NaNO3 as nitrogen source. Eleven isolates of F. oxysporum fsp. cubense have been isolated from several cultivars of banana. Vegetative compatibility tests showed that of 11 eleven isolates, there were 6 different group VCGs where four of them formed volatile compound in rice medium whereas two of them did not. Key words: Fusarium wilt, F. oxysporum fsp. cubense, vegetative compatibility groups (VCGs