Deteksi antigen beredar dalam serum penderita filariasis malayi dengan cara Sandwich ELISA menggunakan antibodi monoklonal Fes1

ABSTRACT Background. The living lymphatic filarial worm Brugia malayi releases physiologically protein materials in the malayan filariasis patients which is called circulating antigen. Based on the clinical symptoms and the demonstration of microfilariae in the blood smears, people who are living in the endemic area can be classified into 4 groups: symptomatic microfilaraemic, symptomatic amicrofilaraemic, asymptomatic microfilaraemic and asymptomatic amicrofilaraemic. Objectives. To detect circulating antigen in sera of each group of patients with malayan filariasis in endemic area. Methods. Circulating antigen in sera was detected by sandwich ELISA using monoclonal antibody Fesi. Result. By sandwich ELISA using monoclonal antibody Fesi, 100% of symptomatic microfilaraemic sera, 35.7% of symptomatic amicrofilaraemic sera, 89.2% of asymptomatic microfilaraemic sera and 26.3% of asymptomatic amicrofilaraemic sera were positive for circulating antigen. Conclusion. Detection of circulating antigen in sera of malayan filariasis patients could be used to support the diagnosis of malayan filariasis in some cases which were difficult to be diagnosed by conventional method based on clinical symptoms and the demonstration of microfilariae in the blood smears. Keywords: circulating antigen - sandwich ELISA - monoclonal antibody - sera - Brugia malayi - asymptomatic amicrofilaraemic. Latar belakang penelitian. Antigen beredar di dalam serum penderita filariasis malayi merupakan protein yang dihasilkan cacing Brugia malayi pada waktu masih hidup di dalam inangnya dan dilepaskan secara fisiologis ke lingkungan sekitarnya. Penderita filariasis malayi di daerah endemik berdasarkan hasil pemeriksaan klinis maupun mikrofilaria di dalam darah dapat dikelompokkan menjadi 4 kelompok yaitu: mikrofilaremia simtomatik, amikrofilaremia simtomatik, mikrofilaremia asimtomatik dan amikrofilaremia asimtomatik. Tujuan penelitian. Mendeteksi antigen beredar di dalam serum masing-masing kelompok penderita filariasis malayi di daerah endemik. Behan dan cara penelitian. Deteksi antigen beredar di dalam serum dilakukan dengan cara sandwich ELISA menggunakan antibodi monoklonal Fesi. Hash! penelitian. Antigen beredar di dalam serum penderita filariasis malayi dapat dideteksi dengan antibodi monoklonal Fes,. Antigen beredar di dalam serum kelompok mikrofilaremia simtomatik terdeteksi 100%, kelompok amikrofilaremia simtomatik terdeteksi 35,7