UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN SIRSAK (ANNONA MURICATA LINN) TERHADAP DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZIL)

AbstractHas done research with the title of Soursop Leaf Extract Antioxidant Activity Assay (Annona muricata Linn) against DPPH (1,1diphenyl-2-picrylhydrazyl). Soursop including the annual plant that can grow and bear fruit throughout the year. This plant is known besides the fruit, leaves are also commonly used as a drug. Soursop leaf is often used empirically in the treatment of cancer (a disease one caused by free radicals / oxidants), so we want to know the antioxidant activity of the soursop leaves. This research aims to determine IC50 value of soursop leaf extract. Test the power of antioxidants in this research was conducted by DPPH (1,1-diphenyl-2-picrylhydrazyl) by spectrophotometry. Extracts has been used in this research is crude extract and extract fractions with different solvents, namely methanol, n-butanol, ethyl acetate, and n-hexane. The results showed that the methanol extract IC50 value is 23,6 ppm, the extract fraction of 30,1 ppm n-hexane, ethyl acetate extract fraction of 18,05 ppm, and n-butanol extract fractions of 14,8 ppm. From these results it can be seen that the soursop leaf extract has a very strong antioxidant activity. Keyword : annona muricata linn, antioxidant, DPPH, IC50, soursop lea