Induction of Mesenchymal Stem Cells from Pluripotent Stem Cells

研究成果の概要（和文): 新規間葉系幹細胞（MSC）ソースの探索を目的に, 多能性幹細胞からのMSCの誘導と評価を実施した. SCIDマウス内でのテラトーマ形成を介したin vivoでの分化誘導, 低酸素培養によるin vitroでの誘導両方法により, 典型的なMSCの形質を示す多能性幹細胞由来MSC様細胞（pcMSC）が得られた. In vitro誘導pcMSCは移植後の腫瘍形成性が認められず, 軟骨再生に寄与した. しかし, in vivo誘導pcMSCにおいては継代培養により高い腫瘍原性が出現した. 以上より, 多能性幹細胞は有望なMSCソースとなりうるが, 腫瘍原性などが異なる場合があるため慎重な検討を要するという結論が得られた. 研究成果の概要（英文): In this study, we derived the MSCs from rabbit ESCs and human iPSCs both in vivo and in vitro, and examined their detailed characteristics. Both in vivo- and in vitro-pcMSCs expressed MSC-markers and showed multiple differentiation properties. In the cases of the in vitro-pcMSCs, teratoma formations were not observed, and when rabbit ESC-derived in vitro-pcMSCs were transplanted into the model rabbits, successful engraftment were observed. On the other hand, the in vivo-induced MSCs derived from human iPSCs showed high telomerase activity and formed teratomas. We could obtain the MSC-like cells both in vivo and in vitro. However, the present results suggest that pluripotent stem cells could derive various types of MSCs, and we should select the cells carefully based on the appropriate markers for our purposes.研究種目：若手研究(B); 研究期間：2011～2013; 課題番号：23791670; 研究分野：医歯薬学; 科研費の分科・細目：外科系臨床医学 整形外科