〈Original〉Identification of Bemisia tabaci biotypes and detection of TYLCV from diseased tomato plants and viruliferous biotype Q whiteflies

[Synopsis] A typical yellow leaf curl disease severely occurred in tomato plants of our greenhouses. The symptom was similar to the disease caused by tomato yellow leaf curl virus (TYLCV). To identify a causal pathogen, tomato plants showing the typical symptom and whiteflies on the plants were tested for their viruliferousness with a commercial TYLCV detection kit. All tomato plants and whiteflies tested were positive for infection with TYLCV. Moreover, biotypes of collected whiteflies were examined using the same PCR method. The present survey revealed that 31 whiteflies were biotype B and five whiteflies were biotype Q of Bemisia tabaci