Comparison of the electrophoresis patterns of myosin light chain from the muscle of developing fish species and Alaska pollack

Alaska pollack, Theragra chalcogramma, is the main fish used in the production of frozen minced fish meat (reitou surimi) in Japan. However, because international regulation of fishing for Alaska pollack is becoming rigorous more and more, the Japanese fishing industry is planning to use other fish for surimi, as well. It may become necessary to be able to distinguish between raw fish for this new surimi from Alaska pollack. Here, we examined whether certain other fishes suitable for use in surimi can be distinguished from Alaska pollack by comparison of the SDS-PAGE pattern of myosin light chain prepared from their surimi. The SDS-PAGE patterns of hoki, Macruronus novaezelanidae; southern blue whiting, Micromesistius australis; Kingklip, Genypterus capensis; Nile perch, Lates niloticus; Chile jurel, Trachurus murphyi; dog shark, Mustlus griseus; requiem shark, Triakis scyllia; Japanese gray shark, Hemitriakis Japanica; and sting-ray, Dasyatis akajei were different, especily for myosin light chain-3, from the patterns from Alaska pollack. Accordingly, discrimination between other raw fish in surimi and Alaska pollack is possible by comparison of the SDS-PAGE patterns of myosin light chain prepared from the surimi.記事区分：原